Chinese hamster ovary (CHO) cells are considered as the most commonly used host for industrial manufacturing of therapeutic proteins. The aim of this study was to evaluate the minimum inhibitory concentration (MIC) of hygromycin B in both CHO-DG44 and CHO-S cells since hygromycin B resistance cassette can be used for future selection of gene expression in CHO cells. The minimum inhibitory conce...

The purpose of this study was to examine the effect of carbohydrate (CHO) augmentation on endurance performance and substrate utilization in aerobically trained women. Eight endurance-trained women completed a 24.2-km (15 mile) self-paced treadmill performance run under three conditions: CHO supplementation (S), CHO loading and supplementation (L+S), and placebo (P). Dietary CHO was approximate...

recently, cho et al. [y. j. cho, r. saadati, s. h. wang, common xed point theorems on generalized distance in ordered cone metric spaces, comput. math. appl. 61 (2011) 1254-1260] dened the concept of the c-distance in a cone metric space and proved some xed point theorems on c-distance. in this paper, we prove some new xed point and common xed point theorems by using the distance in ordere...

a:4:{s:10:"Background";s:897:"Human luteinizing hormone (hLH) belongs to glycoprotein hormones which is composed of two non-covalently linked subunit, α and β. The α-subunit is similar in all glycoprotein hormones, whereas the β-subunit is conferring the hormonal specificity. This hormone has important roles in the growth and maturity of sexual organs and secondary sexual characteristics and st...

Recently, Cho et al. [Y. J. Cho, R. Saadati, S. H. Wang, Common xed point theorems on generalized distance in ordered cone metric spaces, Comput. Math. Appl. 61 (2011) 1254-1260] dened the concept of the c-distance in a cone metric space and proved some xed point theorems on c-distance. In this paper, we prove some new xed point and common xed point theorems by using the distance in ordered con...

The purpose of this study was to determine whether aspirin (A) ingestion combined with prolonged exercise increases gastrointestinal permeability and whether consumption of a carbohydrate-containing (CHO) or a CHO + glutamine-containing (CHO+G) beverage would reduce this effect. Seventeen subjects completed six experiments. They ingested A (1,300 mg) or placebo (P) pills the evening before and ...

Phosphatidylglycerophosphate (PGP) synthase catalyzes the first step in the cardiolipin (CL) branch of phospholipid biosynthesis in mammalian cells. In this study, we isolated a Chinese hamster ovary (CHO) cDNA encoding a putative protein similar in sequence to the yeast PGS1 gene product, PGP synthase. The gene for the isolated CHO cDNA was named PGS1. Expression of the CHO PGS1 cDNA in CHO-K1...

Proteinase 3(PR3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (ANCA) found in Wegener's granulomatosis (WG). We developed a stable expression system for conformationally intact recombinant PR3 (rPR3) in Chinese hamster ovary cells (CHO-cells). The part of PR3 cDNA that encoded the active form of PR3 was s...