background: the regenerative capacity of the mammalian heart is quite limited. recent reports have focused on reprogramming mesenchymal stem cells into cardiomyocytes. we investigated whether fibroblasts could transdifferentiate into myocardium. methods: mouse embryonic fibroblasts were treated with trichostatin a (tsa) and 5-aza-2-deoxycytidine (5-aza-dc). the treated cells were permeabilized ...

objective(s): this study aimed to find out whether the chick cardiomyocyte micromass (mm) system could be employed to predict the teratogenecity of common environmental factors. different multivitamins and over the counter drugs were used in this study.   materials and methods: white leghorn 5-day-old embryo hearts were dissected and trypsinized to produce a cardiomyocyte cell suspension in dul...

background: co-supplementation of unsaturated fatty acids (fas) with saturated fas may decrease the adverse effects of saturated fa-induced lipotoxicity. the objective of the present study was to evaluate the effect of palmitoleic acid (unsaturated fatty acid) on palmitic acid (saturated fatty acid) induced lipotoxicity criteria in the primary culture of adult rat cardiomyocytes. methods: cells...

Background: The regenerative capacity of the mammalian heart is quite limited. Recent reports have focused on reprogramming mesenchymal stem cells into cardiomyocytes. We investigated whether fibroblasts could transdifferentiate into myocardium.Methods: Mouse embryonic fibroblasts were treated with Trichostatin A (TSA) and 5-Aza-2-Deoxycytidine (5-aza-dC). The treated cells were permeabilized w...

introduction: doxorubicin is frequently used for treatment of several types of cancer. doxorubicin cardiac toxicity has limited the use of this drug. corticosteroids may prevent doxorubicin induced cardiotoxicity. therefore the aim of this study was to evaluate mouse embryonic stem cells derived cardiomyocytes as a model to evaluate the effect of doxorubicin and dexamethasone. methods: mouse em...

AIMS The strategies that control formation of the ventricular wall during heart development are not well understood. In previous studies, we documented IGF2 as a major mitogenic signal that controls ventricular cardiomyocyte proliferation and chamber wall expansion. Our objective in this study was to define the tissue source of IGF2 in heart development and the upstream pathways that control it...

Immature contractile cardiomyocytes proliferate to rapidly increase cell number, establishing cardiomyocyte endowment in the perinatal period. Developmental changes in cellular maturation, size and attrition further contribute to cardiac anatomy. These physiological processes occur concomitant with a changing hormonal environment as the fetus prepares itself for the transition to extrauterine l...

introduction: stem cell biology has been the subject of much recent discussion. embryonic stem (es) cells, derived from the inner cell mass of the blastocyst stage of early mammalian embryos are expected to become a powerful tool in future regenerative medicine and developmental biology due to their capacity of self-renewal and pluripotency. in the present study, the ultrastructural development...

background and aims: although metal and metal oxide nanoparticles are used in different medical applications, they may have considerable toxicity on various cells, such as myocytes. therefore, this study aimed to evaluate the toxicity of the naked and serum-treated silver nanoparticles (ag nps) and magnesium oxide nanoparticles (mgo nps) on the cardiomyocytes. materials and methods: cardiomyocy...

One of the striking differences that distinguish the adult from the embryonic heart in mammals and set it apart from the heart in urodeles and teleosts is the incapacity of cardiomyocytes to respond to damage by proliferation. While the molecular reasons underlying these characteristics still await elucidation, mounting evidence collected over the last several years indicates that cardiomyocyte...