C35 was reported to be a new biomarker and therapeutic target for breast cancer. To explore the functional importance of C35, we constructed small interfering RNA (siRNA) targeting C35 and investigated the effects of the siRNAs on C35 expression and apoptosis of T47D cells. C35 siRNAs were constructed and named psiRNA-C35-1 and psiRNA-C35-2. Reverse transcription-polymerase chain reaction (RT-P...

The aim of this study was to investigate the expression of C35, an oncogene previously found in breast and prostate cancers, and its clinicopathological significance in colorectal cancer (CRC). Qualitative and quantitative detection of C35 mRNA expression was performed using reverse transcription-PCR (RT-PCR) and real-time PCR. C35 protein expression was determined using immunohistochemistry. C...

BACKGROUND Resistance to human epidermal growth factor receptor-2 (HER2)-targeted therapies is common, and results in treatment failure and new tumor progression. C35 is over-expressed in many invasive breast cancer endogenously, and functions as an oncogene in breast cancer cell lines. METHODS The current study aims to investigate effects of C35 on cell viability and migration of HER2-positi...

C35 is a novel tumor biomarker associated with metastasis progression. To investigate the interaction factors of C35 in its high expressed breast cancer cell lines, we constructed bait recombinant plasmids of C35 gene and T47D cell cDNA library for yeast two-hybrid screening. Full length C35 sequences were subcloned using RT-PCR from cDNA template extracted from T47D cells. Based on functional ...

Upon coexpression with Erwinia geranylgeranyldiphosphate (GGDP) synthase in Escherichia coli, C(30) carotenoid synthase CrtM from Staphylococcus aureus produces novel carotenoids with the asymmetrical C(35) backbone. The products of condensation of farnesyldiphosphate and GDP, C(35) structures comprise 40 to 60% of total carotenoid accumulated. Carotene desaturases and carotene cyclases from C(...

The aim of this study was to show the possibility of the classification of structural timber (European spruce) into strength class C35 according to EN 338 by using combined visual and machine strength grading techniques. Three different visual machine strength grading combinations are devised for allocating sawn timber into strength class C35. They are composed on the one hand of the visual gra...

Heptaprenyl pyrophosphate synthetase was detected in partially purified extracts of Bacillus subtilis. The enzyme catalyzed the synthesis of all-trans C35 prenyl pyrophosphate from isopentenyl pyrophosphate and farnesyl or geranylgeranyl pyrophosphate, but it did not catalyze a reaction between isopentenyl pyrophosphate and either dimethylallyl or geranyl pyrophosphate. The enzyme reaction proc...

In this work, an evaluation of corrosion resistance boride layers formed on carbon steel C35 in different solutions H2SO4, HCl, HNO3, NaCl and NaOH was carried out. Boriding treatment applied using Ekabor powder at 950 °C for 4 h. The behaviour analyzed by the weight loss method after total immersion tests variable times from 24 hours to 6 months. rate has been determined electrochemical polari...

Se evaluaron los biomarcadores en la fracción de hidrocarburos saturados (n-alcanos, pristano, fitano, terpanos y esteranos) un ensayo biorremediación 90 días con crudo extrapesado (10 ºAPI). Con base presencia una muestra compleja no resuelta (MCNR), deficiencia n-alcanos, fitano hopanos desmetilados (25-norhopanos), se determinó que el original está biodegradado. La detección pristano (más se...