cryopreservation and sexing of embryos are integrated into commercial embryo transfer technologies. to improve the effectiveness of vitrification of in vitro produced buffalo embryos, two experiments were conducted. the first evaluated the effect of exposure time (2 and 3 min) and developmental stage (morula and blastocysts) on the viability and development of vitrified buffalo embryos. morphol...

Buffalo holds the unique potentiality among the livestock sector at tropical and subtropical area. The faster multiplication of superior genotypes and the conservation of endangered buffalo breeds are the important issue. Recent advances in assisted reproductive technologies (ART), including estrus/ovulation synchronization, multiple ovulation and embryo transfer (MOET), in vitro embryo product...

Cryopreservation and sexing of embryos are integrated into commercial embryo transfer technologies. To improve the effectiveness of vitrification of in vitro produced buffalo embryos, two experiments were conducted. The first evaluated the effect of exposure time (2 and 3 min) and developmental stage (morula and blastocysts) on the viability and development of vitrified buffalo embryos. Morphol...

This study was conducted to investigate the effects of capacitating agents added at in vitro fertilization (IVF) and antioxidants supplemented during in vitro culture (IVC) on the development of buffalo embryos. In experiment I, in vitro embryo development of buffalo embryos was compared when the IVF medium was supplemented with heparin, caffeine and calcium ionophore A23187 either alone or in ...

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...

This study was undertaken to determine whether fully grown oocytes isolated from the early antral follicles (2-8 mm size) of frozen-thawed buffalo ovaries are viable and can be rescued to undergo maturation, fertilization, and embryo development in vitro. Ovaries were cryopreserved just after being collected during slaughter from the local abattoir using in situ oocyte (ISO) cryopreservation. I...

The effect of supplementation of follicular fluid (FF) at 5.0% concentration in in vitro maturation (IVM) media on in vitro production of buffalo embryos was studied. Oocytes were aspirated from slaughter house derived ovaries and graded. Cultutarable quality oocytes were randomly cultured in IVM media with FF (n = 334) and without FF (n = 228) and subjected to maturation. The maturation rate, ...

In vitro embryo production (IVP) can help alleviating reproductive difficulties in cattle and buffalo. This study compared qualitative quantitative features of native buffalo ovaries oocytes to understand them comprehensively. addition, it was conducted pick the ideal for oocyte collection assess either maturation affects diameter or not. The were collected from local slaughterhouses Dhaka city...

In this paper, an account of various aspects related to buffalo reproduction are given. Fundamental concepts of the reproductive physiology as well as manipulation of the reproductive function will be presented. This will include an overview of the most recent developments of the oestrous cycle and the ovulation control, new strategies of reproductive management for the improvement of genetic g...

Reproductive biotechnologies continue to be developed for genetic improvement of both river and swamp buffalo. Although artificial insemination using frozen semen emerged some decades back, there are still considerable limitations. The major problem appears to be the lack of efficient methods for estrus detection and timely insemination. Controlled breeding experiments in the buffalo had been l...