In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (...

Aim: The experiment was conducted to study the total seminal plasma protein (TSPP) and heparin-binding proteins (HBPs) in relation to initial semen quality of buffalo bull. Materials and Methods: Semen from two Murrah buffalo bulls (bull no. 605 and 790) with mass motility of ≥3+ were used for the study and categorized into three groups (Group IMass motility 3+, Group IIMass motility 4+ and Gro...

Different semen assays were employed to determine the post -thaw fitness of buffalo and Sahiwal bull spermatozoa. Semen (two ejaculates) was collected from buffalo and Sahiwal bulls (six each) once a week for three consecutive weeks. Semen was diluted in lactose-fructose-egg yolk glycerol extender, equilibrated at 4 ac for 4 hr and frozen in liquid nitrogen vapors. A highly significant differen...

The study was designed to assess the effects and optimal range of osmotic pressure of citrate egg yolk extender (CEYE) for cryopreservation of buffalo bull semen. Semen samples of four Nili-Ravi buffalo bulls, kept for artificial insemination (AI) purposes, were collected at weekly interval for five weeks. Five solutions of tri-sodium citrate dihydrate and D (-) fructose of varying osmotic pres...

This study was carried out to determine if a new antibiotic combination comprising of gentamycin, tylosin and linco-spectin (GTLS) in extender is suitable for improvement in fertility of liquid buffalo bull semen through artificial insemination (AI). Two consecutive ejaculates per week (4 weeks) were collected from three Nili-Ravi buffalo bulls of known fertility by using artificial vagina (42°...

Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the maj...

semen quality and its relationship to fertility are major concern in animal production. the aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through artificial insemination(ai). semen samples were collected from four buffalo bulls. semen volume and sperm concentration appeared to be significantly different (p0...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

in vitro fertility assessment using fluorescent technique is a better predictor of fertility status of bulls as compared to traditional semen quality assessment techniques, therefore, the study was planned to assess in vitro fertility status of bulls based on conventional and fluorescent techniques. seventy-three ejaculates were collected from 12 murrah buffalo bulls maintained at artificial br...

Buffalo spermatozoa have been studied cytochemically and biochemically to investigate their phospholipids, which appear to be phosphatidyl choline, lysolecithin, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl glycerol and monogalactosyl diglyceride. Most of them form lipoprotein complexes in the regions of the plasma membrane, the post-nuclear cap and the mid-piece. The lipid co...