Background: Since the sheep ‘Dolly’ was successfully cloned by somatic cell nuclear transfer (SCNT), the inefficiency of SCNT cloning and abnormality of cloned animals have been a focus of considerable controversy. Oocyte quality has been shown to contribute to poor somatic cloning and cell reprogramming efficiency with many factors affecting oocyte quality and developmental potential. It has b...

Background: The most important factor affecting the efficiency of somatic cell nuclear transfer (SCNT) is the initial quality of the oocyte. Since the abattoir-derived oocytes are heterogeneous in quality, selection of cohort fully grown/competent oocytes is necessary for proper development of SCNT embryos. Materials and Methods: Glucose-6-phosphate dehydrogenase (G6PDH) is actively expressed i...

sawdust which is the main waste from wood industry has been used as a raw material forremoval of brilliant cresyl blue toxic dye. the effects of various parameters such as ph,electrolyte concentration, adsorbent dose, contact time and agitation rate were studied for theremoval of the dye in a concentration of 50 mg l-1. the optimum condition for the studiedparameters was applied for various con...

Brilliant blue is largely found in ice cream, canned processed peas, packet soups, blue raspberry flavored products, dairy products, sweets and drinks. It is also used in soaps, shampoos, mouthwash and other hygiene and cosmetics applications (Archived from Florida). This study was aimed to investigate the antimicrobial effects of Brilliant blue on Bacillus subtilis as a gram positive bacterial...

a novel, simple and more sensitive spectrophotometric procedure has been developed for the determination of brilliant blue fcf and carmoisine in food samples by an aqueous two-phase system (atps). after extraction, the absorbance values were measured at 527 and 632 nm for carmoisine and brilliant blue fcf, respectively. the effects of different parameters such as, polyethylene glycol (type and ...

A novel, simple and more sensitive spectrophotometric procedure has been developed for the determination of Brilliant blue FCF and Carmoisine in food samples by an aqueous two-phase system (ATPS). After extraction, the absorbance values were measured at 527 and 632 nm for Carmoisine and Brilliant blue FCF, respectively. The effects of different parameters such as, polyethylene glycol (type and ...

The covalent immobilization of synthetic or natural macromolecular compounds containing amino groups onto polystyrene (PS) solid surfaces is of great interest in diagnostic applications. A sensitive assay allowing the determination of reactive end groups is therefore a powerful tool for predicting the performance of the active surface. Recently, we reported the use of the Coomassie brilliant bl...

Previous studies have confirmed that oxidized adenosine triphosphate, a P2X7 receptor antagonist, attenuates lipopolysaccharide-mediated microglial activation and inflammatory expression following neuronal damage in rat brain. NaCl and temperature may affect the potency of oxidized adenosine triphosphate. Brilliant blue G is a derivative of a widely used food additive and has little toxicity. T...

A simple analytical procedure is proposed for simultaneous determination of three common dyes (Basic Blue 9, Brilliant Blue E-4BA, and Reactive Blue 2) in natural waters without prior separation of the solutes. A popular chemometric method, partial least squares regression PLS-1, was effectively applied for spectral resolution of a highly overlapping system. At the best modeling conditions, mea...