the holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (bola-drb3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp). genomic dna was extracted from bull semen using phenol-chloroform method. a two-step pcr was conducted in order to amplify a 284 base-pair fragment of the target gene. amplicons were digested by rsai, haeiii and bstyi ...

The Holstein bulls (n=50) were genotyped for bovine lymphocyte antigen (BoLA-DRB3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Genomic DNA was extracted from bull semen using phenol-chloroform method. A two-step PCR was conducted in order to amplify a 284 base-pair fragment of the target gene. Amplicons were digested by RsaI, HaeIII and BstyI ...

Bovine gamma/delta T cells and neutrophils roll on 24 h cytokine- or lipopolysaccharide-stimulated bovine fetal umbilical cord endothelial cells in assays done under physiological flow. An antibody directed against E- and L-selectin has minimal blocking effect on this rolling interaction. mAbs were raised against the stimulated bovine endothelial cells and screened for inhibition of gamma/delta...

Immature dendritic cells (DCs) resident in bovine spleens represent a distinct CD11a(+) CD11c(+) CD13(+) CD172(+) CD205(+) population compared to those circulating in peripheral blood or trafficking via afferent lymph. Upon cytokine-induced maturation, splenic DCs both efficiently present antigen in the stimulation of allogeneic lymphocyte proliferation and recall antigen-specific responses.

Recently, dysfunction of antigen-specific T cells is well documented as T-cell exhaustion and has been defined by the loss of effector functions during chronic infections and cancer in human. The exhausted T cells are characterized phenotypically by the surface expression of immunoinhibitory receptors, such as programmed death 1 (PD-1), lymphocyte activation gene 3 (LAG-3), T-cell immunoglobuli...

The in vitro expression of bovine leukemia virus (BLV) in short-term cultured bovine peripheral blood mononuclear cells (PBMC) is associated with increased spontaneous lymphocyte blastogenesis. The purpose of this study was to determine whether intracellular pathways responsible for antigen- or mitogen-induced lymphocyte blastogenesis were also responsible for induction of BLV expression. The p...

patients and methods in this cross-sectional study, peripheral mononuclear cells (pbmcs) were isolated from peripheral blood of 29 patients with newly diagnosed pulmonary tb and 19 patients with positive tuberculin skin test. the pbmcs were activated with ppd for 72 hours. activated cells were harvested, rna was extracted and cdna was synthesized. a real-time taqman method was designed and opti...

unresponsiveness to hepatitis b surface antigen (hbsag) has been shown to be associated with dysfunction of the presenting cells (apc) and defect in the specific b-lymphocyte and/or t-lymphocyte repertoires.   direct determination of the frequency of specific t-lymphocytes together with complementary analysis of the naive circulating immune cells could provide valuable information about the cel...

Experimental bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus (BLV) were performed on sheep. BIV proviral DNA and anti-BIV antibodies were persistently detected in all BIV-inoculated sheep. A slight increase in lymphocyte counts was observed in BIV-infected sheep, but the percentages of CD4+ and CD8+ cells in sheep peripheral blood mononucl...