dialysis-related amyloidosis (dra) is characterized by accumulation of amyloid β2-microglobulin (β2m) in the interstitial matrix. matrix substances such as heparin have reportedly been strongly implicated in the pathogenesis of dialysis-related amyloidosis. in clinical setting of hemodialysis, two types of heparin, i.e., high and low molecular heparin (h.m.h. and l.m.h.) have been routinely use...

RNA-binding proteins often contain multiple domains connected by short flexible linkers. This domain arrangement allows the protein to bind RNA with greater affinity and specificity than would be possible individual sometimes remodel its structure. It is therefore important understand how modules interact because it modular nature of these which specifies their biological function. chapter conc...

Biolayer interferometry is a method to analyze protein interactions in real-time. In this study, we illustrate the usefulness to quantitatively analyze high affinity protein ligand interactions employing a kinetic titration series for characterizing the interactions between two pairs of interaction patterns, in particular immunoglobulin G and protein G B1 as well as scFv IC16 and amyloid beta (...

The purpose of this white paper is to compare four techniques utilized to quantify biomolecular interactions. It provides a brief introduction to each technique, followed by tables that provide a direct comparison. The four techniques compared in this white paper are Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Microscale Thermophoresis (MST), and Biolayer Interferom...

1. General Experimental Details 2. Oxidative Addition Complexes and Synthetic Procedures 3. Peptide Synthesis and LC-MS Characterisation 4. Macrocyclisation Reactions and LC-MS Characterisation 5. C-CA Protein Expression 6. BioLayer Interferometry Binding 7. Lipophilicity, Phospholipid Affinity, Volume of Distribution, and Human Serum Albumin Binding Data 8. ICP-MS Analysis 9. References 10. NM...

The performance of an antibody in an assay often correlates with its affinity to the antigen. The ability to specifically select for antibodies on the basis of binding strength is therefore an important element of antibody assay development. Affinity or koff-rate determination can be carried out on several platforms; for example a rough ranking by ELISA, or quantitative screening using a techno...

Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based qua...