This work demonstrates the first example of the immobilisation of MAO-N whole cells to produce a biocatalyst that remained suitable for repetitive use after 11 months of storage and stable up to 15 months after immobilisation. The production ofEscherichia coli expressing recombinant MAON was scaled up to bioreactors under regulated, previously optimised conditions (10 % DO, pH 7), and the amoun...

BACKGROUND Biocatalyst improvement through molecular and recombinant means should be complemented with efficient process design to facilitate process feasibility and improve process economics. This study focused on understanding the bioprocess limitations to identify factors that impact the expression of the terminal hydroxylase CYP153A6 and also influence the biocatalytic transformation of n-o...

BACKGROUND The bacterium Acetobacter sp. CCTCC M209061 is a promising whole-cell biocatalyst with exclusive anti-Prelog stereoselectivity for the reduction of prochiral ketones that can be used to make valuable chiral alcohols such as (R)-4-(trimethylsilyl)-3-butyn-2-ol. Although it has promising catalytic properties, its stability and reusability are relatively poor compared to other biocataly...

alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. the ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. a. borkumensis have two genetic systems for alkane biodegradation. the first system is alkane hydroxylase (alk-b1and alk-b2) and the second system is  cytochrome p450....

The vanadium-dependent chloroperoxidase from Curvularia inaequalis is a stable and efficient biocatalyst for the hydroxyhalogenation of a broad range of alkenes into halohydrins. Up to 1 200 000 TON with 69 s-1 TOF were observed for the biocatalyst. A bienzymatic cascade to yield epoxides as reaction products is presented.

The microbial fuel cell (MFC) is an ecologically friendly alternative energy source. Due to the typically limited electron transfer in MFC systems, co-biocatalysts are necessary enhance their performance. Enzymes used as due superior ability generate energy, and system known enzymatic (EMFC). One of substrates that may be bagasse waste extracted from sugarcane. Saccharo­myces cerevisiae enzyme ...

Industrial biocatalysis has been identified as one of the key enabling technologies that, together with transition to continuous processing, offers prospects for development cost-efficient manufacturing high-quality products and low waste generation. This feature article highlights role miniaturized flow reactors free enzymes cells in success this endeavor recent examples their use single or mu...

At this short review, different chemical production of 3D graphene biocatalysts and developing of its characters by new substituted for using in enzymatic fuel cells are investigated. Also, the current ways of production of 3D Graphene Biocatalysts, different types of substitutes, the best methods for having the highest efficiency, the physical, chemical and biological characters of new biocata...