background: there are only four drugs for treating african trypanosomiasis, a devastating disease in sub-saharan africa. with slow discovery of better drugs, vaccination is viewed as the best method of control. we previously showed that antibodies to native trypanosoma brucei brucei tubulin inhibit the growth of trypanosomes in culture. here, we aimed to determine the effect of antibodies to ba...

background: in order to deworm the ruminants especially of sheep in iran, consumption of benzimi­dazoles has more than 2 decades history and today farmers are using imidazothiazoles, macrocyclic lactones and mostly benzimidazole compounds (bzs) to treat infected farm animals. it has been demonstrated that the most common molecular mechanism leading to bzsresistance in haemonchus contortus is a ...

The organization of the alpha- and beta-tubulin gene families in Physarum was investigated by Mendelian analysis. Restriction endonuclease-generated DNA fragments homologous to alpha- and beta-tubulin show length polymorphisms that can be used as markers for genetic mapping. Analysis of meiotic assortment among progeny of heterozygotes allowed alpha- and beta-tubulin sequence loci to be defined...

background and objectives: a new real- time pcr protocol for the detection of trichomonas vaginalis in pooled urine samples has been optimized and validated. materials and methods: the amplification protocol, targeting a 2kb repeated gene in the t. vaginalis genome, was optimized by varying pcr parameters. as a reference method, a real-time pcr protocol targeting the beta-tubulin gene (y. versl...

We have cloned two different beta-tubulin sequences from the filamentous fungus Aspergillus nidulans. Each was used in the construction of transforming plasmids that carry the pyr4 gene of Neurospora crassa. We used these plasmids to transform a pyrG-strain of Aspergillus to uridine prototrophy. Both plasmids were shown to integrate site specifically into the homologous chromosomal sequences. W...

We have previously demonstrated that the chicken genome contains at least four different, functional beta-tubulin genes. By using gene specific probes we have now analyzed the relative levels of expression of the four encoded messenger RNA (mRNA) transcripts as a function of chicken development and differentiation. We have found that the RNA transcript from the beta 2 gene is present in large a...

During cell division and during the induction of tubulin synthesis that accompanies flagellar regeneration in Chlamydomonas reinhardi, four tubulin mRNAs of discrete molecular sizes are produced. During induction two beta tubulin mRNAs (2.47 kb and 2.34 kb) and two alpha tubulin mRNAs (2.26 kb and 2.13 kb) are synthesized in high abundance and in a closely coordinated fashion. Combined data fro...

An altered beta-tubulin gene that confers resistance to benomyl [whose active ingredient is 2-(methoxycarbonylamino)benzimidazole (MBC)] was isolated from a DNA library of Aspergillus flavus and used as a selectable marker for transformation. The beta-tubulin gene was cloned into a plasmid vector containing the pyr-4 gene of Neurospora crassa, and transformants were selected either for uracil p...