to develop an elisa technique for the detection of antibodies against babesia ovis, the infectederythrocytes were lysed and the supernatant soluble antigen, after sonication and ultracentrifugation of thelysate was used as antigen. optimal dilution of the antigen was determined by checkerboard titrations, usingpositive and negative control sera. a correlation of 85% was observed between the res...

To develop an ELISA technique for the detection of antibodies against Babesia ovis, the infectederythrocytes were lysed and the supernatant soluble antigen, after sonication and ultracentrifugation of thelysate was used as antigen. Optimal dilution of the antigen was determined by checkerboard titrations, usingpositive and negative control sera. A correlation of 85% was observed between the res...

In this study, the prevalence of piroplasms in sheep and goats was assessed with Giemsa-stained blood smear examination, PCR and nested PCR-restriction fragment length polymorphism (RFLP) to identify Babesia and Theileria species, respectively, in 338 small ruminants (172 sheep and 166 goats) from three sites in North-West Tunisia during the 2011 summer season. The overall infection prevalence ...

background: ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in iran caused by babesia ovis, b. motasi, and b. crassa . the aim of this study was to characterize the species of ovine babesia species isolated from different geographical region of iran. methods: one hundred fifty four blood samples collected from animals, which demonstrated the pale muco...

BACKGROUND Ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in Iran caused by Babesia ovis, B. motasi, and B. crassa. The aim of this study was to characterize the species of ovine Babesia species isolated from different geographical region of Iran. METHODS One hundred fifty four blood samples collected from animals, which demonstrated the pale mucou...

BACKGROUND Small ruminants' babesiosis caused by Babesia ovis, is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. This study was carried out to to estimate the infection rate of B. ovis in sheep and goats by PCR. We have analysed risk factors that might influence infection of sheep and goats with B. ...

background: babesia ovis and theileria ovis are among the important and main etiological agents causing ovine babesiosis and ovine theileriosis, causing severe economic losses among sheep and goats. the aim of the present study was to determine the prevalence and molecular diagnosis of b. ovis and t. ovis in lohi sheep at livestock experiment station bahadurnagar, okara, pakistan. methods: the ...

this study was conducted to determine the electrophoretic pattern of serum protein in goats infected withbabesia ovis. serum total protein was calculated and serum electrophoresis from 15 goats naturally infectedwith b. ovis as well as same number of healthy goats was performed. microscopic examination of giemsastained peripheral blood smears revealed b. ovis infection. the parasitological diag...

Background: Babesia is blood-tissue parasite, which are transmitted by hard ticks from Ixodidae family. The parasite is the cause of Babesiosis among ruminants. Babesia is as one of main fatal factors among livestock in endemic regions such as Iran. The aim of this study was to investigate Babesia spp infection using microscopic and molecular methods among small ruminants in Mazandaran and Gole...

The prevalence of Babesia spp. infection was studied in sheep of the Mashhad area in Iran from 1998 to 2000. A total of 677 sheep originating from 115 flocks were clinically examined and investigated for the presence of Babesia spp. in appropriate blood smears and any tick species on the body of the animals. The study revealed that the infection rate for Babesia ovis and Babesia motasi were 167...