in this study, the immunogenicity of a rabies vaccine that was used in one of the isfahan cattle farms is reported. this vaccine was produced by replication of the pasteur virus (pv) strain on bhk-21 monolayer cell culture. the virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. thus, the obtained vaccine was tested on mice and a sat...

Compared to other methods of vaccine production, (e.g. culture flask and bioreactor system), the roller culture method for producing rabies vaccine is simpler, and the equipment easier to install and maintain, and also in the event of contamination of one roller, the entire batch need not be discarded. This system provides a much larger surface area than the culture flask, and also ensures a mu...

In this study, the immunogenicity of a rabies vaccine that was used in one of the Isfahan cattle farms is reported. This vaccine was produced by replication of the Pasteur virus (PV) strain on BHK-21 monolayer cell culture. The virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. Thus, the obtained vaccine was tested on mice and a sat...

compared to other methods of vaccine production, (e.g. culture flask and bioreactor system), the roller culture method for producing rabies vaccine is simpler, and the equipment easier to install and maintain, and also in the event of contamination of one roller, the entire batch need not be discarded. this system provides a much larger surface area than the culture flask, and also ensures a mu...

rabies is acutely fatal encephalitis caused by a neurotropic virus. this virus belongs to the family ofrhabdoviridae and genus of lyssavirus. the virus is almost always transmitted to human through infectedmammalian saliva. rabies is inoculated to a wound by an infected animal bite. since infection is establishedin the cns, the outcome is almost always fatal. according to the outstanding role o...

Mice challenged with rabies virus were vaccinated 24 h later with one of two types of rabies vaccine. Vaccine prepared from a BHK cell substrate resulted in the production of serum interferon and neutralizing antibody, whereas vaccine prepared from a human diploid cell substrate gave rise to neutralizing antibody but no interferon. Only the first vaccine was effective in reducing mortality. Var...

Mice challenged with rabies virus were vaccinated 24 h later with one of two types of rabies vaccine. Vaccine prepared from a BHK cell substrate resulted in the production of serum interferon and neutralizing antibody, whereas vaccine prepared from a human diploid cell substrate gave rise to neutralizing antibody but no interferon. Only the first vaccine was effective in reducing mortality. Var...

Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...

background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...

A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of Afri...