A novel approach to the presumptive screening of questioned semen stains has been developed which enables the rapid identification of stains which are devoid of semen. Questioned semen stains can be swabbed with a moist cotton swab, and the prostatic acid phosphatase (SAP) activity transferred to the swab identified through assay with 5-bromo-4-chloro-3-indolyl phosphate (BCIP). Controlled labo...

Breast cancer is a disease with high heterogeneity. Many issues on tumorigenesis and progression are still elusive. It is critical to identify genes that play important roles in the progression of tumors, especially for tumors with poor prognosis such as basal-like breast cancer and tumors in very young women. To facilitate the identification of potential regulatory or driver genes, we present ...

The determination of gene expression patterns in three dimensions with cellular resolution is an important goal in developmental biology. However the most sensitive, efficient, and widely used staining technique for whole-mount in situ hybridization (WMISH), nitroblue tetrazolium (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) precipitation by alkaline phosphatase, could not yet be combined w...

the slow development. The low background especially applies for detection of digoxigenin-labeled RNA probes. When detecting identical probes labeled with fluorescein, the background is higher, presumably because of nonspecific binding of the fluorescein antibody (the higher background is detected with both BCIP and NBT/BCIP). In cases in which the investigated signals are of different intensity...

In prior studies, models of inflammatory pain were produced through injecting complete Freund's adjuvant (CFA) or capsaicin directly into either the deep somatic tissue or the animal's hind paw. In contrast, bone cancer-induced pain (BCIP) was simulated through injecting tumor cells into the cavity of the femur or the tibia. It has been reported that, due to differences in afferent innervation,...

In this study, a novel paper-based SERRS immunoassay based on magnetic separation and alkaline phosphatase (ALP) enzyme catalyzed hydrolysis reaction was developed. By using modified antibodies conjugated to magnetic beads, capture of mouse IgG followed by addition of ALP-labeled antibodies would form a sandwich-like immunoconjugate. After magnetic separation, 5-bromo-4-chloro-3-indolyl phospha...

Homochiral crystallizations of two enantiomeric metal-organic frameworks (MOFs) Ce-MDIP1 and Ce-MDIP2 were achieved by using L- or D-BCIP as chiral inductions, respectively, where the chiralities were characterized by solid state CD spectra. Ce-MDIPs exhibit excellent catalytic activity and high enantioselectivity for the asymmetric cyanosilylation of aromatic aldehydes; the homochiral Cd-TBT M...

General. Solid-phase peptide synthesis was carried out in plastic syringe reactors. Fmocprotected amino acids were obtained from Novabiochem (Läufelfingen, Switzerland) or prepared according to standard procedures. Bovine serum albumin (A7638), biotinylated WGA (L5142), alkaline phosphatase-coupled monoclonal anti biotin antibody (A6561), BCIP/NBT tablets (B5655), peroxidase-labeled WGA (L7017)...

In situ hybridization techniques typically employ chromogenic staining by enzymatic amplification to detect domains of gene expression. We demonstrate the previously unreported near infrared (NIR) fluorescence of the dark purple stain formed from the commonly used chromogens, nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP). The solid reaction product has significant...