In this short review, we discuss apolipoprotein B100 and the assembly of very low-density lipoproteins. In particular, we address the nature and importance of co- and posttranslational degradation of apolipoprotein B100 during the assembly process. We also provide a short historical background to the development of the current model for the degradation of apolipoprotein B100.

Apo-E-deficient apo-B100-only mice (APOE:(-/-)APOB:(100/100)) and LDL receptor-deficient apo-B100-only mice (LDLR:(-/-)APOB:(100/100)) have similar total plasma cholesterol levels, but nearly all of the plasma cholesterol in the former animals is packaged in VLDL particles, whereas, in the latter, plasma cholesterol is found in smaller LDL particles. We compared the apo-B100-containing lipoprot...

The present study analyzes the heat release and combustion characteristics of diesel, pure biodiesel (B100) obtained from Jatropha oil and its 80% blending with Ethanol (BD80E20) and Acetone (BD80AC20) as fuels in a single cylinder four stroke direct injection (DI) diesel engine. The analyses of experimental results indicate that the pressure of the in-cylinder gases rise early in case of B100,...

Addition of sodium butyrate to the culture medium of the human hepatoma cell line Hep G2 resulted in a time- and dose-dependent increase in the secretion of apolipoprotein A-I (apo A-I) and apolipoprotein B100 (apo B100). After a 24 h preincubation period, a 2.4- and 2.2-fold increase in the secretion of apo A-I and apo B100 respectively was obtained during the next 24 h in the presence of 2 mM...

OBJECTIVE Anderson disease is a rare inherited lipid malabsorption syndrome associated with hypocholesterolemia and linked to SAR1B mutations. The aim of this article was to analyze the mechanisms responsible for the low plasma apolipoprotein Apo-B100 and Apo-AI in 2 patients with Anderson disease. METHODS AND RESULTS A primed constant infusion of (13)C-leucine was administered for 14 hours t...

All classes of lipoproteins considered to be atherogenic contain apo-B100 or apo-B48. However, there is a distinct paucity of data regarding whether lipoproteins containing apo-B48 or apo-B100 differ in their intrinsic ability to promote the development of atherosclerosis. To address this issue, we compared the extent of atherosclerosis in three groups of animals: apo-E-deficient mice (apo-B+/+...

In this study, mustard biodiesel (B100) was produced from low quality crude mustard oil and tested in a four-cylinder, direct-injection, diesel engine to investigate the combustion, performance and emission characteristics of the engine at different engine speeds and full load conditions. Biodiesel and its blends showed increased peak cylinder pressure and reduced ignition delay when compared t...

PURPOSE OF REVIEW Adaptive immune responses have been shown to play an important role in the atherosclerotic disease process and both pathogenic and protective immunity has been identified. Apolipoprotein (apo) B100 appears to be a key antigen and novel therapies modulating immune responses against apo B100 have shown promising results in experimental models. This review will discuss recent dev...

three centrifugations (gel I). Rather, we believe that successive centrifugation of large chylomicrons for 6 x 10' gmin results in their purification with loss of VLDL and small chylomicrons containing apo B100. The results demonstrate that purified large chylomicrons contain apo B-48, but do not contain apo B100 and that the apo B-1 00 in chyle is located in the small chylomicron and VLDL frac...

Insulin regulates hepatic VLDL production by activation of phosphatidylinositide 3-kinase (PI3-kinase) which decreases apo B available for lipid assembly. The current study evaluated the dependence of the VLDL apolipoprotein B (apo B) pathway on PI3-kinase activity in vivo. VLDL production was examined in B100 only, apo B mRNA editing catalytic subunit 1 (apobec-1(-/-)) mice, using the Triton W...