in journal: 1. Bálványos, I., László, I., Vida, K., Hank, H., Kursinszki, L., Tóth, E., Sz ke, É.(2001): Effect of magnesium on the genetically modified Atropa belladonna cultures. Magnesium Research 14, 309-310. IF: 0,689 2. Hank H., Bálványos I., Kursinszki L., Tóth E., Sz ke É. (2003): A táptalaj összetételének hatása transzgénikus Atropa belladonna kultúrák alkaloid produkciójára. Gyógyszer...

Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6β-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna. All of t...

Putrescine N-methyltransferase (PMT) is the first alkaloid-specific enzyme for nicotine and tropane alkaloid formation. The pmt gene from Nicotiana tabacum was fused to the CaMV 35S promoter and integrated into the Atropa belladonna genome. Transgenic plants and derived root cultures were analysed for gene expression and for levels of alkaloids and their precursors. Scopolamine, hyoscyamine, tr...

The medicinally applied tropane alkaloids hyoscyamine and scopolamine are produced in Atropa belladonna L. and in a small number of other Solanaceae. Calystegines are nortropane alkaloids that derive from a branching point in the tropane alkaloid biosynthetic pathway. In A. belladonna root cultures, calystegine molar concentration is 2-fold higher than that of hyoscyamine and scopolamine. In th...

background & aim: a. belladonna (family: solanaceae) is one of important pharmaceutical plants which contain tropane ‎alkaloids. tropane alkaloids are distinct group of secondary metabolites of the ‎solanaceae family.  the most important alkaloids of a. belladonna are atropine and hyoscine that are used extensively because of their medicinal properties. therefore identification and ‎determi...

The tropane alkaloids, hyoscyamine and scopolamine, are medicinal compounds that are the active components of several therapeutics. Hyoscyamine and scopolamine are synthesized in the roots of specific genera of the Solanaceae in a multistep pathway that is only partially elucidated. To facilitate greater understanding of tropane alkaloid biosynthesis, a de novo transcriptome assembly was develo...

■ ■ UPC2 method compatible with mass spectrometry for identification of unknown components IN T RODUC T ION The extract of Atropa belladonna, a perennial native to south and central Europe, North Africa, and East Asia has been used for centuries to treat a number of medical conditions, including ulcers and other gastrointestinal disorders, and pain. It is also used as an anti-muscarinic agent. ...

In this overview we explore the electroanalytical determination of molecule murder: atropine. Atropine, occurs naturally in various plants nightshade family, including deadly (Atropa belladonna). On one hand, atropine, a tropane alkaloid, has medical uses, named on World Health Organisations list essential medicines, used for example resuscitations and as an antidote to certain poison gases ins...

Plants are a potential source of a large number of valuable secondary metabolites. In vitro cultures are being considered as an alternative to agricultural processes for studying valuable secondary metabolites. In this way, nutritive factors are important parameters influencing the production of these compounds in plants. Effects of nitrate concentrations (KNO3) on the production of two tropane...

BACKGROUND Atropa belladonna (Engl. deadly nightshade, Cro. velebilje, bunika) is a plant containing pharmacologically active, potentially toxic alkaloids: atropine, hyocyamine and scopolamine. The risk of poisoning in children is important because of possible confusion of black/dark blue belladonna fruit berries with other edible berries. There are many reports in literature of accidental into...