EFFECTS OF CARBOHYDRATE SOURCE ON THE IN VITRO ASYMBIOTIC SEED GERMINATION OF THE TERRESTRIAL ORCHID HABENARIA MACROCERATITIS Scott L. Stewart a b & Michael E. Kane a a Plant Restoration, Conservation, and Propagation Biotechnology Program, Environmental Horticulture Department, University of Florida, Gainesville, Florida, USA b Horticulture and Agriculture Programs, Kankakee Community College,...

BACKGROUND AND AIMS Ecotypic differentiation has been explored in numerous plant species, but has been largely ignored in the Orchidaceae. Applying a specific germination protocol for widespread seed sources may be unreliable due to inherent physiological or genetic differences in localized populations. It is crucial to determine whether ecotypic differentiation exists for restoration and conse...

Our study was to develop a rapid and successful method for promoting asymbiotic seed germination and growth and development of protocorm like bodies (PLBs) and subsequent plantlets of Phalaenopsis orchid. Four basal media i.e. Murashige and Skoog (MS), half strength MS (½MS), Knudson C (KC) and, Vacin and Went (VW) supplemented with coconut water and peptone were examined for their effectivenes...

Abstract: Asymbiotic germination techniques are successful in species of the Catasetum genus. The diverse composition culture media can be critical; however, viable seeds required for asymbiotic germination. reliable method viability assessment is tetrazolium test. A decisive step efficiency test preconditioning with sucrose solution, which has potential to improve Therefore, this work aimed ev...

In vitro asymbiotic seed germination of Dendrobium chrysotoxum varied with fruit harvesting time and culture medium used for germinating seeds. Seeds at different developmental stages were harvested and cultured on five asymbiotic orchid seed germination media i.e. defined and undefined media, namely 1⁄2 MS, KC, M, MS and PDA. Seeds harvested 220 days after pollination showed maximum germinatio...

In the context of a symbiotic plant-fungus interaction study concerning Cattleya purpurata, we focused on some aspects seed morphology and biology, early stages seedling development. Seed was characterized using light scanning electron microscopy. vitro germination capability evaluated, comparing asymbiotic methods. The seeds overall comparable to that other congeneric species, showing classica...

Procedures for asymbiotic germination and seedling development under in vitro ex conditions were investigated Bletilla striata. Five different media (½ P6668 ¬Phytamax Orchid Maintenance Media, ½ Media with coconut water, P723 PhytoTechnology Seed Sowing MS media) culture sphagnum moss examined their effectiveness 8 weeks on seed of B. Germination occurred all media, however, the best rate was ...

five different media with two concentrations and kinds of carbohydrates and peptone treatments were examined for their effectiveness in promoting asymbiotic seed germination of epipactis  veratrifolia. results revealed significant differences between media on seed germination percentage and protocorm growth and fast medium has the best effect on both. carbohydrate treatments had significant eff...