objective(s): apolipoprotein e genotype (apoe) polymorphism affects lipid levels and coronary artery disease (cad) risk. the aim of this study was to study the association of the apolipoprotein e genotypes with coronary artery disease in the iranian population.   materials and methods: the apolipoprotein e genotype in dna samples extracted from 66 cad+ patients and 61 control subjects by restri...

We present a modification to the polymerase chain reaction amplification/Hhal restriction isotyping method for human apolipoprotein (apo) E. This method includes a mutagenic forward primer and 5'-end labeling of both primers. These modifications of the original method described by Hixon and Vernier (J Lipid Res 1990;31:545-8) allow sensitive and unambiguous determination of apoE genotypes.

poproteins in mouse peritoneal macrophages. J Mol Med 2000;78:217–27. 3. Wenham PR, Sedky A, Spooner RJ. Apolipoprotein E phenotyping: a word of caution. Ann Clin Biochem 1991;28:599–605. 4. Hixson JE, Vernier DT. Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI. J Lipid Res 1990;31:545–8. 5. Somsen GW, Welten HT, Mulder FP, Swart CW, Kema IP, de Jong...

In patients with dementia, 29 diagnosed as probably suffering from Alzheimer's disease and 46 subjects with dementia of vascular origin, and in 41 non demented control subjects LDL oxidation in vitro was compared in carriers of various apolipoprotein E alleles. Restriction isotyping was performed by gene amplification and cleavage with Hhal, LDL oxidation was investigated by determination of co...

  Objective(s): Apolipoprotein E genotype (APOE) polymorphism affects lipid levels and coronary artery disease (CAD) risk. The aim of this study was to study the association of the Apolipoprotein E genotypes with coronary artery disease in the Iranian population.   Materials and Methods: The Apolipoprotein E genotype in DNA samples extracted from 66 CAD+ patients and 61 control s...

The genotyping of the various isoforms of Apolipoprotein E (apo E) has been performed using matrix-assisted laser desorption/ionization (MALDI-MS). The polymerase chain reaction was used to amplify the specific apo E gene sequence followed by digestion with Cfo I (Clostridium formicoaceticum), for generating restriction fragments for rapid and accurate mass analysis. An exonuclease I digestion ...

Apolipoprotein E (apoE) genotypes were determined in a random subset of 1041 subjects enrolled in the Framingham Offspring Study by using DNA amplification followed by restriction isotyping. The results were compared with the apoE phenotypes previously assessed by isoelectric focusing. Discrepancies in apoE allele assignment were found in 98 subjects (9.4%). Both genotype and phenotype were rea...

Variations at the apolipoprotein E (APOE) locus influence lipid and lipoprotein levels in the normal population, and are associated with premature coronary artery disease. In the present study, the APOE genotypes and allele frequencies in 299 women from Novosibirsk (West Siberia) were determined. APOE genotypes were detected by restriction isotyping after amplification of the genomic DNA by PCR...