نتایج جستجو برای: Antibiotic marker free strains
تعداد نتایج: 848093 فیلتر نتایج به سال:
a new system is presented for the generation ofrecombinant bacillus subtilis strains without antibioticmarkers. this system is based on two plasmids constructed in escherichia coli. the first plasmid phm30contains an incomplete hisi gene, the last gene in thehistidine biosynthesis operon of b. subtilis and part ofthe genes yvca and yvcb of unkown function flankinghisi at the 3´-end. the spectin...
A new system is presented for the generation of recombinant <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt...
World population growth and requirement to global food security, application of genetic engineering and utilization of transgenic organisms have made more important. Using this technology, without regarding to its risks, can cause loses to environment. To generate transgenic organisms, selection systems are applied that cause to selective growth of transformed cells. Antibiotic resistance genes...
Background and purpose: Rotational use of antibiotics can decrease antibiotic resistance in Staphylococcus aureus strains. The present study aimed to evaluate the alteration of oxacillin resistance in methicillin-resistant Staphylococcus aureus (MRSA) strain carrying SCCmec III cassette in long-term evolutionary experiments in an antibiotic-free medium. Materials and methods: In a cross-sectio...
background spirulina is one of the most profitable known microalgae in the world, which is used as food and superfood. in the other hand, spirulina is a useful source of healthy components. it seems that the spirulina is transformable so that the introduction of a selectable marker is needed. objectives the purpose of this study was to determine a suitable selection marker for spirulina platens...
Antibiotic selectable marker genes have been widely used to generate transgenic animals. Once transgenic animals have been obtained, the selectable marker is no longer necessary but raises public concerns regarding biological safety. The aim of this study was to prepare competent antibiotic selectable marker free transgenic cells for somatic cell nuclear transfer (SCNT). PhiC31 intergrase was u...
Enterobacteriaceae bacteria are clinically important and lead to various diseases associated with high mortality morbidity, including urinary tract infections, yet their treatment is a challenge due increasing antibiotic resistance. This study examined the cytotoxicity effects viability-inducing abilities of mecillinam fosfomycin antibiotics in extended-spectrum beta-lactamase Klebsiella pneumo...
Background: Listeriosis can be fatal for vulnerable groups of society. The disease has been widespread in recent years due to the large consumption of dairy and meat products. There is little information about the susceptibility of antibiotics and the pattern of Listeria monostigenesis gene resistance in Iranian society. Accordingly, the present study was conducted to investigate the antibiotic...
Allelic exchange is frequently used in bacteria to generate knockout mutants in genes of interest, to carry out phenotypic analysis and learn about their function. Frequently, understanding of gene function in complex processes such as pathogenesis requires the generation of multiple mutant strains. In Pseudomonads and other non-Enterobacteriaceae, this is a time-consuming and laborious process...
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