background helicobacter pylori (h. pylori) is one of the most common infectious diseases in the world. it colonizes about 50-60% of the world’s population. the estimated prevalence of h. pylori infection is approximately 65% in iran. objectives the aim of the present study was to investigate the antimicrobial resistance of h. pylori to metronidazole, clarithromycin and amoxicillin. materials an...

Reports on the isolation of amoxicillin-resistant Helicobacter pylori are increasing worldwide, which may cause serious problems in eradication therapy. To elucidate the mechanism of amoxicillin resistance of H. pylori, penicillin-binding proteins (PBPs) of amoxicillin-resistant strains isolated in Korea were analysed. Three PBPs (66, 63 and 60 kDa) were identified in both amoxicillin-resistant...

OBJECTIVES To elucidate the relationship between the mutations of penicillin-binding protein (PBP)1, PBP2 and PBP3 and amoxicillin resistance in Helicobacter pylori. METHODS The mutations detected only in clinical amoxicillin-resistant strains were determined by comparison of the deduced amino acid sequences of PBP1(HP0597), PBP2(HP1556) and PBP3(HP1565) encoded by the pbp1, ftsI and pbp2 gen...

To investigate amoxicillin and metronidazole resistance of Helicobacter pylori, we compared putative resistance genes between resistant strains obtained in vitro and their sensitive parent strain. All metronidazole-resistant strains had rdxA mutations, and an amoxicillin-resistant strain had pbp1 and pbp2 mutations. By transforming PCR products of these mutated genes into antibiotic-sensitive s...

The present study aimed to evaluate the antibiotic resistance pattern of Escherichia coli strains isolatedfrom animals to ascertain the levels of antibiotic resistance pervasiveness. A total of 28 E. coli strains wereisolated from faecal samples and the antibiotic resistance pattern of E. coli strains was determined by meansof disc diffusion assay. The resistance pattern determined for all stra...

BACKGROUND/AIMS A worldwide increase in amoxicillin resistance in Helicobacter pylori is having an adverse effect on eradication therapy. In this study, we investigated the mechanism of the amoxicillin resistance of H. pylori in terms of amino acid substitutions in penicillin-binding protein 1 (PBP1). METHODS In total, 150 H. pylori strains were isolated from 144 patients with chronic gastrit...

Most Helicobacter pylori strains are susceptible to amoxicillin, an important component of combination therapies for H. pylori eradication. The isolation and initial characterization of the first reported stable amoxicillin-resistant clinical H. pylori isolate (the Hardenberg strain) have been published previously, but the underlying resistance mechanism was not described. Here we present evide...

The aims of this study were to assess primary resistance of H. pylori strains isolated from adult patients of Ilam, Iran to antibacterial agents (amoxicillin, clarithromycin, metronidazole and tetracycline) and detection of clarithromycin, azithromycin, clarithromycin, metronidazole and tetracycline resistance by disc diffusion. Fifty biopsies were taken from gastric mucosa of the antrum and bo...

the present study aimed to evaluate the antibiotic resistance pattern of escherichia coli strains isolatedfrom animals to ascertain the levels of antibiotic resistance pervasiveness. a total of 28 e. coli strains wereisolated from faecal samples and the antibiotic resistance pattern of e. coli strains was determined by meansof disc diffusion assay. the resistance pattern determined for all stra...

The aim of the study was to isolate gram-positive cocci from cows with mastitis and to determine their resistance to beta-lactamic antibiotics. Eight hundred and nine strains were isolated and identified as staphylococci (n=516), streptococci (n=199) and enterococci (n=94) from sub-clinical and clinical cases of bovine mastitis in Lithuania. The most common causative agents of udder disease inc...