Exogenous estrogens or pollutants with estrogen-like activity can induce vitellogenin (VTG) synthesis in male and juvenile fish, making this protein a useful indicator of chemicals that mimic estrogenic activity. The purpose of this study was to investigate the impact of municipal wastewater on blood biochemical parameters of common carp (Cyprinus carpio). Under experimental conditions, biomark...

Complement factor C3, the central protein of the complement system, was found to be phosphorylated both in EDTA- and heparin-anticoagulated whole blood and in coagulating blood. Complement S protein (vitronectin) was also found to be phosphorylated under these conditions. Further, purified C3 was found to be a phosphoprotein in vivo, containing 0.15 mol of alkali-labile phosphate/mol of protein...

determine the molecular basis for the cytotoxic action that produces the antitumor effect. Unfortunately, there is pres ently no direct way to identify with certainty the critical molecular lesions. It is, however, possible to obtain comrel ative evidence between molecular effects and cytotoxic effects in various cell types, which could lead to a probable identification of the critical lesions....

Rat liver acetyl-CoA carboxylase has been purified to homogeneity by a new method involving polyethylene glycol precipitation, and DEAE and Sepharose 4B chromatography. The final product displays a single band on SDS polyacrylamide gel electrophoresis of estimated molecular weight 240,000. This material contains 5.5 +/- 0.3 moles of alkali-labile phosphate per subunit and has a specific activit...

Each of more than 30 monoclonal antibodies that had been raised against Mycobacterium leprae and previously classified as reactive with carbohydrate was shown to be directed against lipoarabinomannan, a prominent, highly pervasive, myo-inositol-phosphate-containing, cross-reactive antigen within the leprosy bacillus. Some of the antibodies preferentially bound to the lipopolysaccharide of M. le...

The validity of the reducing end group (copper reduction) method for determination of chain weight of chondroitin sulfate is shown by comparison with chain weight determination from linkage region components, xylose and galactose. It is established that chains of both chondroitin sulfate and keratan sulfate are stable to alkali as strong as 2.0 N NaOH at room temperature for at least 120 hours....

information about forms of phosphorus (p) is important for the evaluation of p status in soil and also understanding of the soil fertility and plant nutrition. to obtain such information and to determine the relationship between the p forms from soil characteristics, amount of different organic and inorganic p forms of 20 soil composite samples of eastern azerbaijan province, iran were determin...

Fructosamine 3-kinase, which phosphorylates low-molecular-mass and protein-bound fructosamines on the third carbon of their deoxyfructose moiety, is quite active in erythrocytes, and was proposed to initiate a process removing fructosamine residues from proteins. In the present study, we show that incubation of human erythrocytes with 200 mM glucose not only caused the progressive formation of ...

The N-acetylgalactosamine in the walls of Bacillus subtilis 168 occurs in two polymers. One of these contains N-acetylgalactosamine, glucose and phosphorus and is attached to the peptidoglycan through an alkali-labile bond; preliminary studies indicate that a repeating unit of this polymer is glucosyl-N-acetylgalactosamine 1-phosphate. N-Acetylgalactosamine is also associated with the peptidogl...

In 0.05 M potassium phosphate buffer (pH 7.0, ZO”), native rabbit muscle aldolase has been found to exhibit a molecular weight (mn N g, pZ) close to 160,000, in agreement with previous findings. Upon exposure to cold alkaline borate buffer (pH 12.5, p = 0.17, 0”), the enzyme spontaneously dissociates into its monomers which subsequently undergo slow hydrolytic degradation. Compensating for elec...