Azotobacter vinelandii stimulated the growth of Agrobacterium tumefaciens H2, H23, H24, H27, and ATCC 15955 on media containing insoluble iron sources. The Azotobacter vinelandii siderophores appeared to promote Agrobacterium tumefaciens growth by solubilizing mineral iron, and the ferrisiderophores so formed then acted as iron sources for Agrobacterium tumefaciens. Agrobactin, the Agrobacteriu...

Agrobacterium: Microbe Wiki http://microbewiki.kenyon.edu/index.php/Agrobacterium This website provides an excellent overview of the genus Agrobacterium. It contains photos and links to additional information. Genome of Agrobacterium tumefaciens http://www.genomenewsnetwork.org/articles/12_01/ A_tumefaciens_genome.shtml A. tumefaciens has been the most widely studied and used Agrobacterium spec...

Ethylene evolution from plants inhibits Agrobacterium-mediated genetic transformation, but the mechanism is little understood. In this study, the possible role of ethylene in Agrobacterium-mediated genetic transformation was clarified. It was tested whether or not plant ethylene sensitivity affected genetic transformation; the sensitivity might regulate bacterial growth during co-cultivation an...

Rhizobium, Agrobacterium and Allorhizobium are genera within the bacterial family Rhizobiaceae, together with Sinorhizobium. The species of Agrobacterium, Agrobacterium tumefaciens (syn. Agrobacterium radiobacter), Agrobacterium rhizogenes, Agrobacterium rubi and Agrobacterium vitis, together with Allorhizobium undicola, form a monophyletic group with all Rhizobium species, based on comparative...

Agrobacterium tumefaciens has a unique ability to transfer genes into plant genomes. This ability has been utilized for plant genetic engineering. However, the efficiency is not sufficient for all plant species. Several studies have shown that ethylene decreased the Agrobacterium-mediated transformation frequency. Thus, A. tumefaciens with an ability to suppress ethylene evolution would increas...

Root nodulating bacteria was isolated from root nodules of Vigna trilobata (L) Verdc. Plants raised in the soils were collected from five geographically different areas in Andhra Pradesh, India. The bacteria was identified as Agrobacterium tumefaciens strains by 16S rDNA analysis. Except A. tumefaciens MRR 102, all the strains produced round shape and white coloured colonies with lot of mucilag...

An efficient protocol of common bean transformation using Agrobacterium tumefaciens is still unavailable. Several regeneration reports are available using cotyledonary node or embryonic axis as the starting explants. These explants were used for direct organogenesis exploiting the meristems for direct shoot production. Attempts were made to transform cotyledonary node explants and embryonic axe...

agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. this gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. in the present work, we studied maize transformation using a. tumefaciens by identifying som...

Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer. To suppress ethylene evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into Agrobacterium tumefaciens. The ACC deaminase enhanced A. tumefaciens-mediated gene transfer into plants.

Scientific achievement in molecular biology and genetic engineering have made it possible to create new methods of selection k that are based on genome modification. Todays, more genetically modified plants being intensively tested the field, but their influence has not yet been fully studied environment, biodiversity, human health, animal body.
 The article analyzes results research prese...