objective(s): microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   materials and methods: in this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-l-lysine (pll). some of the aldehyde groups of the activated agaro...

Agarose-vinyl sulfone (VS) beads have proven to be a good support immobilize several enzymes. However, some enzymes are hardly immobilized on it. This is the case of penicillin G acylase (PGA) from Escherichia coli, which very slowly this (less than 10% in 24 h). enzyme also not significantly adsorbed aminated MANAE-agarose beads, an anionic exchanger. In study, were modified with divinyl (DVS)...

conclusions the pe gene was successfully cloned and confirmed by sequencing. finally, pe was obtained with high concentration. due to high homology and similarity among the pe gene from nthi atcc 49766 and other nthi strains in genbank, we believe that the protein is a universal antigen to be used as a vaccine design candidate and further studies to evaluate its immunogenicity is underway. mate...

Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...

background: the purpose of this study was to evaluate the dependency of magica polymer gel dosimeter response (r2) on different electron energies as well as on different mean dose rate for a standard clinically used linear accelerator. materials and methods: the sensitivity of the dosimeter was represented by the slope of calibration curve in the linear region measured for each modality. a cali...

Vinyl sulfone (VS)–agarose beads were used to develop a new strategy coimmobilize enzymes with very different stabilities, enabling the reuse of most stable immobilized enzyme. Two model combi-biocatalysts prepared. First, trypsin and chymotrypsin multipoint covalently coimmobilized on VS–agarose, support was blocked ethylenediamine, then β-galactosidase from Aspergillus oryzae via anion exchan...