background: leishmaniases are a group of diseases caused by leishmania parasites .  growing of drug unresponsiveness in leishmaniasis patients necessitates the development of new drugs and accor­dingly a suitable assay is needed for evaluation of any modalities.  the aim of this study was to compare four drug assays methods, agar dilution, broth dilution, cylinder plate and disk diffusion, for ...

helicobacter pylori is the causative agent of gastritis and peptic ulcer disease. eradication of h. pylori is hard to achieve and often require multiple antibiotics regimens. these regimens are quite expensive and have substantial side effects. licorice is a traditional drug which is frequently used for gastric discomforts in iran. the susceptibility of h. pylori to the extract of licorice has ...

OBJECTIVES Tentative standards for testing MICs for Mycobacterium tuberculosis include agar dilution and the BACTEC method. However, the conventional agar dilution method requires 3-5 weeks to complete; whereas BACTEC, although a rapid test, involves the use of radioisotopes. In contrast, the MGIT 960 system uses a fluorescence quenching based oxygen sensor that can be read automatically. This ...

Echinocandins are a group of antifungal agents that target 1,3-beta-glucan synthase, causing disruption of mold growth at cells compromising tips and branch points. In part because echinocandins do not induce clear growth inhibition end points using broth dilution techniques, methods to test susceptibility have not yet been standardized. We developed a novel susceptibility assay that measures g...

BACKGROUND Leishmaniases are a group of diseases caused by Leishmania parasites. Growing of drug unresponsiveness in leishmaniasis patients necessitates the development of new drugs and accordingly a suitable assay is needed for evaluation of any modalities. The aim of this study was to compare four drug assays methods, agar dilution, broth dilution, cylinder plate and disk diffusion, for evalu...

There are currently no standardized phenotypic methods for the screening and detection of AmpC enzymes. This study aimed to evaluate different methods to detect AmpC enzymes in Escherichia coli, Klebsiella spp., and Proteus spp., comparing the results from two disk-based methods and an agar dilution method. AmpC activity was determined for 255 clinical isolates by use of a three-dimensional enz...

exploration of natural products with antitumoral effects such as etoposide, tenioposide, camptothecin and paclitaxel from conifers provoked us to do some research on anti-tumoral activity of iranian medicinal plants. ten different species of iranian conifers aerial parts were collected across the country. the relative potency of methanolic extract were investigated on the inhibition of topoisom...

In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such...

A urea broth microdilution method to assay the susceptibilities of seven Malassezia species was developed. This method indicated the same sensitivities as the agar plate dilution method for isolates of Malassezia furfur, M. pachydermatis, M. slooffiae, and M. sympodialis.