An evaluation of the humoral response based on secretory immunoglobulin A levels in the saliva of pulmonary tuberculosis (TB) acid-fast bacillus-positive (TB-AFB+) patients against a recombinant 38 kDa antigen (Ag38-rec) is reported. A total of 60 saliva samples consist of 30 TB-AFB+ patients and 30 healthy controls were tested against 500 ng of semi-purified antigen using the dot blot method. ...

We studied the immune response of salivary secretory Objective immunoglobulin A (sIg-A) from a pediatric tuberculosis (TB) group (scoring > and =6) and non-TB group (scoring <6) against recombinant Ag38 (Ag38-rec) from Indonesian strain. Mycobacterium tuberculosis : Seventy-eight children were divided into three Materials and Methods groups; those with TB (n=26), those with suspected TB (n=26),...

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. The α-mangostin in mangosteen pericarp extract can inhibit M. tuberculosis growth. This study examined the potency of α-mangostin in mangosteen pericarp extract to inhibit 38-kDa and Ag85 protein secretion from M. tuberculosis H37Rv. The samples used in this study were divided into three independent variable groups...

An approach to stimulating an immune response against tumors is to transduce tumor cells with bacterial genes, which represent a "danger signal" and can induce a wide immune response. Mycobacterium tuberculosis genes and their encoded proteins play a pivotal role in linking innate and cell-mediated adaptive immunity and represent ideal candidates as immune adjuvants for tumor vaccines. The effi...

Penelitian ini bertujuan untuk mengisolasi protein alergen belut segar (BS) dan rebus (BR) dengan buffer Tris Glisin (TG), DTT (TGD) Phosphat saline (PBS) serta mengkarakterisasi alergenisitas isolat uji elektroforesis SDS-PAGE, ELISA immunoblotting . Rendemen yang dihasilkan pada BSTG, BSTGD BSPBS sebesar 2166 mg/L, 2083 mg/L 2284 mg/L. ekstrak BRTG, BRTGD, BRPBS adalah 1445 1805 1207 Hasil SD...

Phycobiliproteins have been used as a natural dye, an analytical probe for fluorescence studies, and chemical medicinal use. An ultrafiltration membrane is useful the purification of phycobiliproteins. This study addressed phycobiliprotein-containing solution, extracted from Nostoc commune , which was individually performed by two kinds (MWCO 150 kDa 10 kDa) in cross-flow. Three proteins were m...

Production of small-sized peptides is significant because their health benefits. Ultrafiltration (UF) membrane provides an effective fractionation on a large scale. Thus, the present study was aimed to evaluate performance multilayer UF in fractionating tilapia fish by-product (TB) protein hydrolysate by observing permeate flux, peptide transmission, and distribution under different stirring sp...