Liquid chromatography with fluorescence detection using immunoaffinity column clean-up was a method described for determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2) in peanuts and peanut based products. The validation of the procedure was performed. Good coefficient of correlation was found for all aflatoxins in the range of 0.9993–0.9999. Limit of detection (LOD) and limit of quantificati...

This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting so...

Aflatoxins are mycotoxins of major concern to the dairy industry. Most mycotoxins are found in grain, usually grown in a drought, although they may contaminate pasture grass and occasionally hay. Most frequently, aflatoxins are found in corn (see Figures 1-3) and cottonseed, and sometimes in their byproducts, but also on rare occasions in soybeans or distiller’s grain. Peanut products also may ...

Aflatoxins are natural contaminants of food crops implicated in the pathogenesis of various human diseases. This study aimed to determine the associations between aflatoxins and proteinenergy malnutrition (PEM) by measurements of aflatoxins in serum, urine and food on plate of Nigerian children with PEM. A crosssectional study was undertaken in 3 agroecological regions of Nigeria (Guinea savann...

A study was carried out to assess levels of contamination of aflatoxins and fumonisins (B1 + B2) in maize produced, stored and consumed in rural households in Malawi. A total of 9 districts were selected across the country representing 3 districts from each of the Northern, Central and Southern regions respectively. Households were selected at random in each district where 10 maize samples were...

To detect and monitor G-group aflatoxins in agricultural products, we generated class-specific monoclonal antibodies that specifically recognized aflatoxins G₁ and G₂. Of the final three positive and stable hybridomas obtained, clone 2G6 produced a monoclonal antibody that had equal sensitivity to aflatoxins G₁ and G₂, and did not cross-react with aflatoxins B₁, B₂, or M₁. Its IC50 values for a...

Introduction and background Aflatoxins are a hazard in foods because they are potent carcinogens, capable of causing cancer in nearly all animal species studied. Aflatoxins are also acute, chronic and genotoxic poisons (1). In addition, recent evidence suggests strongly that aflatoxins can be immunosuppressive (2). The primary sources of aflatoxins are the common fungi Aspergillus flavus and th...

Aspergillus flavus isolates produce only aflatoxins B1 and B2, while Aspergillus parasiticus and Aspergillus nomius produce aflatoxins B1, B2, G1, and G2. Sequence comparison of the aflatoxin biosynthesis pathway gene cluster upstream from the polyketide synthase gene, pksA, revealed that A. flavus isolates are missing portions of genes (cypA and norB) predicted to encode, respectively, a cytoc...

An interlaboratory study was performed in eight laboratories to validate a liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of aflatoxins and sterigmatocystin (STC) in white rice and sorghum (Sorghum bicolor). Fortified samples (at three different levels) of white rice and sorghum were extracted, purified through a solid-phase extraction (SPE) ...