Monoclonal antibodies specific for aflatoxin B1, aflatoxin B2, aflatoxin M1, and the major aflatoxin-DNA adducts were obtained following fusion of mouse SP-2 myeloma cells with spleen cells of mice immunized with aflatoxin B1 covalently bound to bovine gamma globulin. The aflatoxin-modified protein used to immunize mice was produced chemically by activating aflatoxin B1 to a 2,3-epoxide derivat...

We have examined the time course and patterns of covalent aflatoxin B1:DNA adducts produced within the ribosomal RNA gene sequences isolated from the liver nuclear DNA of aflatoxin B1-treated animals. Liver nuclear DNA was initially enriched in ribosomal DNA by cesium salt density centrifugation, and incubated under alkaline conditions to stabilize bound aflatoxin B1-DNA moieties. Alkali-treate...

Five experimental groups were used to evaluate the efficiency of sodium bentonite (BNT) and ascorbic acid (AA) in reducing the toxicity of aflatoxin B1 in ElSalam chicks (Egyptian local chicks) diets. A total number of 450 unsexed one-day old chicks were wing banded, weighted and randomly assigned to 5 groups of five replicates (18 chicks/replicate). The experimental groups were as follow: 1) b...

The effects of various inducers on the activities of drug-metabolizing enzymes including aflatoxin B1 activation were studied in Syrian golden hamsters. Activity for aflatoxin B1 was determined by aflatoxin B1-DNA adducts formation. The treatments of hamsters with 3-methylcholanthrene, alpha-naphthoflavone and benzo(a)pyrene elevated markedly the activity for aflatoxin B1 by 2460, 1380 and 450%...

A strategy for the detection of aflatoxin B1 using a capacitive biosensor has been studied. The use of proteins for the generation of sites with high specificity against aflatoxin B1 are produced via bioimprinting. This technique has become a tool for the detection of aflatoxin B1 using a capacitive biosensor. The results demonstrate the ability to generate specific interactions with aflatoxin ...

Aflatoxin B1, the most potent hepatic chemical carcinogen known, is activated to the putative product aflatoxin B1-2,3-epoxide via a cytochrome P-450-dependent reaction. Mt. Shasta rainbow trout is the most sensitive species known to the hepatocarcinogenic effects of aflatoxin B1. We have previously isolated and purified a minor form of cytochrome P-450 from this strain of rainbow trout, with a...

AFLATOXIN B1, a carcinogenic metabolite of the fungus Aspergillus flavus, is acutely toxic to a number of species, including rats. Male rats (LD50 7-2 mg./kg.) are more susceptible to the acute oral effects of aflatoxin than are females (LD50 17.9 mg./kg.) (Butler, 1964). Aflatoxin B1 is metabolised to aflatoxin M1 which is present in the blood and urine of dosed rats (Butler and Clifford, 1965...

Extended Abstract Aflatoxin B1 is one of the most common mycotoxins, toxic substances produced by fungi, and produced by Aspergillus flavus. It is classified as one of International Agency for Research on Cancer (IARC) Group 1 carcinogens and only few nanograms of aflatoxin B1 may cause liver cancer and stunted growth by permeation through skin. There are conventional diagnosis methods such as ...

Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and con...

The antibacterial properties of aflatoxin B1 have been evaluated against antibiotic-resistant clinical isolates of Escherichia coli and Staphylococcus aureus. The inhibition of growth ranged from 11.5 to 60.0% and 4.5 to 18.5% in the strains of S. aureus and E. coli, depending on the extent of drug resistance. Aflatoxin-B1 binding varied with toxin concentration, the presence of surfactants (Tw...