in this study escherichia coli de3 containing expression vector (pet21a) with cloned persian sturgeon growth hormone (psgh) gene was grown in 10 ml lb broth on a 150 rpm shaker, at the temperature of 37 °c. at the late log phase (determined by od standard curve) 100 μl isopropyl β-d-1-thiogalactopyranoside (iptg) was added for induction of gh synthesis. samples were taken every 2 hours and afte...

introduction: the major histocompatibility complex (mhc) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. the physiologic function of mhc molecules is the presentation of peptides to t cells. in this study, we evaluated the purification of a class ii mhc molecule (hla-dr) from a human burkitt′s lymphoma cell line; daudi. materi...

background: the ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis. the aim of this study was purification of mouse igg2a and production of polyclonal antibody against purified mouse igg2a subclass. materials and methods: mouse igg2a was purified by proa affinity. verification method of the purified antibody was sds-...

the purification of biomolecules is a necessary step in many biochemical researches. in this regard, developments of convenient, specific and low cost methods of purification are of particular interest. given the human hemoglobin (hb) affinity toward some charged carbohydrates, interaction of this molecule with human chorionic gonadotropin (hcg) which is a glycoprotein hormone containing sialic...

the efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. conclusions were drawn regarding selection of solid phase according to pore size, rigidity, ph stability, chemistry of derivation and activation, and gross concentration of immobilized ligand required fo...

background: plasminogen is one of the compounds derived from human plasma. activation of plasminogen produces plasmin. plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. the aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine sepharose. materials and methods: normal human plasma was used as the start...

Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...

the aim of the present work was to purify cysteine protease inhibitors from rapeseed (brassica napus l.), with potential activity on digestive protease of colorado potato beetle (cpb), leptinotarsa decemlineata (say). ammonium sulfate precipitated proteinaceous fractions; 30, 50, 70, and 100% showed 39.07, 57.03, 51.47, and 22.44% inhibition on the fourth instar larval gut general protease acti...