adenoviruses (advs) types 40 and 41 are the causative agents of diarrhea in children. hence, rapid sensitive and specific detection of these viruses are of clinical importance. the customary methods such as propagation of virus in cell culture suffer from limitations. detection of immobilized amplified products in a one phase system (diapops) method has the potential to overcome these problems....

Adenoviruses (AdVs) types 40 and 41 are the causative agents of diarrhea in children. Hence, rapid sensitive and specific detection of these viruses are of clinical importance. The customary methods such as propagation of virus in cell culture suffer from limitations. Detection of immobilized amplified products in a one phase system (DIAPOPS) method has the potential to overcome these problems....

The prevalence of enteric adenoviruses detected by an in-house enzyme-linked immunosorbent assay (the RIVM-ELISA) ranged from 13 to 38%, and subgroup F adenoviruses comprised 86%. All subgroup F adenoviruses reacted with both RIVM anti-adenovirus type 40 (Ad40) and anti-adenovirus type 41 (Ad41) monoclonal antibodies but were not detected by Adenoclone Type 40/41 enzyme immunoassay (EIA). The c...

We have studied the DNAs of fastidious enteric adenoviruses recovered from the stools of infants with gastroenteritis. By endonuclease analysis, the strains examined represent candidate adenovirus types 40 and 41, which are thought to comprise new adenovirus subgroups F and G. Cloning of DNA from representative enteric adenovirus isolates, together with hybridization and subcleavage analysis, p...

The virus-associated (VA) RNA gene regions of the human subgroup F adenoviruses (types 40 and 41) were amplified using primers corresponding to flanking open reading frames of human and simian (SA7) adenovirus sequences previously published. The subgroup F adeno-viruses, like human Ad12 (subgroup A) and SA7, were found to have only one VA RNA gene at this locus (map unit 30). The type 40 and ty...