Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acro...

Background: Evaluation and comparison of total acrosin activity along with DNA integrity in the semen samples obtained from the varicocele individuals before and after the surgery and also in fertile individuals. Materials and Methods: Semen parameters, protamine deficiency, DNA fragmentation and Total acrosin activity were measured in patients with a clinical diagnosed varicocele of grade II o...

Acrosin activity and semen quality (sperm concentration, ejaculate volume and number of spermatozoa) were assessed from March 1997 to March 1998 in semen of Large White, Pietrain and Duroc x Pietrain boars. Semen quality varied with season, including high production of spermatozoa in autumn and winter and low production in summer. Semen quality also differed across breeds. Acrosin activity of b...

Background: Introduction assessment of sperm functional tests along with routine semen analysis may provide further information in the etiology of varicocele in different subpopulations. Therefore, in this study, in addition to assessment of sperm chromatin integrity, we aimed to assess the total acrosin activity which has proven to have major role during in vitro and natural fertilization in v...

We evaluated the effect of time and temperature on acrosin release from the acrosomal cap and the activity of this enzyme during in vitro capacitation in fresh and frozen/thawed dog sperm. Sperm-rich fractions of six ejaculates from three dogs were processed as fresh and frozen samples. Each sperm sample was incubated in canine capacitation medium (CCM) for 0, 1, 2 and 3 h at 20°C and at 37°C. ...

AIM To study the association between seminal oxidative stress and human sperm acrosin activity. METHODS It is a prospective study consisting of 30 infertile men and 12 fertile normozoospermic volunteers. A full history, clinical examination and scrotal ultrasound were done to exclude other related factors such as smoking and varicocele. Presence of white blood cells (WBCs) in semen samples wa...

AIM To develop a method for assessing sperm function by measuring released acrosin activity during the acrosome reaction (AR). METHODS Human semen samples were obtained from 24 healthy donors with proven fertility after 3-7 days of sexual abstinence. After collection, samples were liquefied for 30 min at room temperature. Standard semen parameters were evaluated according to World Health Orga...

OBJECTIVE To detect the presence of antibodies to the proacrosin/acrosin system and to evaluate their effect on the sperm acrosomal protein activities in women consulting for infertility. DESIGN Retrospective study. SETTING Basic research laboratory. PATIENT(S) Recombinant proteins derived from human proacrosin (Rec-40, Rec-30, Rec-20, Rec-10) and recombinant human zona pellucida (ZP) gly...

Acrosin, a proteolytic enzyme located in the acrosome of sperm, exhibits amidase, esterase, and proteinase activity on synthetic and natural substrates containing arginyl and lysyl residues. Highly purified acrosin preparations from boar acrosomes have endopeptidase activity cleaving only the carboxyl bonds of arginine and lysine with a strong preference for arginine bonds. The Michaelis consta...

The divalent metal cation ionophore A23187 rapidly induces a normal acrosome reaction in a population of guinea-pig sperm suspended in calcium medium. In the course of the acrosome reaction, proacrosin, the zymogen precursor of the protease acrosin, is activated. Although the acrosome reaction causes exocytosis of the acrosomal contents, 'soluble' acrosin is not released in significant amounts ...