The aim of this investigation was to determine the efficacy of chalcopyrite bioleaching using pure cultures of Acidithiobacillus ferrooxidans or Acidithiobacillus thiooxidans and a mixed culture composed of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. The experiments were carried out in shake flasks with [Fe] 4 gL 1 and S 1 gL 1 at pH 1.80, 130 rpm and 30 C. Inocula of both...

Applicability of the manometric method for studying the oxidation of pyritic material in the presence of bacteria has been demonstrated. Resting cells of Ferrobacillus ferrooxidans accelerated the oxidation of coal pyrites and coarsely crystalline marcasite, but were inactive on coarsely crystalline pyrite. Resting cells of Thiobacillus thiooxidans were inactive on all pyrites tested. Oxidation...

Polyphasic genotypic analysis of 25 Acidithiobacillus ferrooxidans strains isolated from ores and ore concentrates collected in different regions of the world showed considerable strain heterogeneity. Restriction patterns of the chromosomal DNA of these strains obtained by PFGE were specific for each strain. According to the degree of DNA relatedness, 17 of the 23 strains studied were divided i...

Acidithiobacillus ferrooxidans ILS-2 was adapted in digested sludge and used to treat for dewaterability improvement. Results showed that increasing ferrous iron loading increased dewaterability, but the inoculation of bioleaching strain had little effect on compared controls without strain. The total extracellular polymeric substances (EPS) contents sludges with treatment were similar except a...

Phospholipids (PLs) are the important components of cell membrane of all living organisms. The PL or the cell membrane of the bacteria are the least studied subject as it involves large quantities of material and few methods to assay. The novel study was designed to understand if the cell membrane remains same or it changes during different conditions. The bacteria, Acidithiobacillus ferrooxida...

Acidithiobacillus ferrooxidans type strain DLC-5, isolated from Wudalianchi in Heihe of Heilongjiang Province, China. Here, we present the draft genome of strain DLC-5 which contains 4,232,149 bp in 2745 contigs with 57.628% GC content and includes 32,719 protein-coding genes and 64 tRNA-encoding genes. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession no. JNNH0000000...

 Cyanidation is a relatively simple and cheap technology to treat gold-containing ores. However, this process is not completely effective in refractory gold-bearing sulfide ores. In the case of very low-grade ores, biooxidation is an attractive alternative pre-treatment to render the gold amenable to extraction. This process utilizes the ability of acidophilic bacteria to oxidize the sulfide m...

the effects of different factors on growth and bio-oxidation efficiency of a native strain of acidithiobacillus ferrooxidans have been evaluated by the utilization of response surface methodology, rsm. medium ph and iron concentration were found to be the most significant factors while temperature and ammonia concentrations had the least weight within the ranges investigated. optimum operationa...

The mining industry is looking forward for bacterial consortia for economic extraction of copper from low-grade ores. The main objective was to determine an optimal bacterial consortium from several bacterial strains to obtain copper from the leach of chalcopyrite. The major native bacterial species involved in the bioleaching of sulphide ore (Acidithiobacillus ferrooxidans, Acidithiobacillus t...

this study deals with bioleaching of the low grade uranium ore of saghand mine in iran using a mixed culture of mesophilic bacteria (acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans and leptospirillum ferrooxidans). experiments were carried out in shaking flask examining the effect of temperature, initial ferrous ion concentration, pulp density and particle size. variations of ph, ...