BACKGROUND Aflatoxins are the most extensively studied group of mycotoxins produced by molds, especially the Aspergillus group, which are highly toxic to animals and humans. OBJECTIVES Since immunoassay is a simple and rapid method for the analysis of many toxic substances in comparison to the chromatographic methods, it is necessary to produce specific and sensitive antibodies for detection ...

AFM1-oxime-Fluoresceinamine conjugate was prepared and chracterized. .For that purpose fist was obtained and purified AFM1-oxime. UV-Vis scan spectral analysis of the obtained AFM1oxime-Fluoresceinamine conjugate was made. The activity of AFM1-oxime-Fluorescenamine conjugate was measured. It was proved that the obtained conjugate had high activity and potential possibility for detection of AFM1...

Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or f...

This study was carried out to detect the presence of aflatoxin M1 (AFM1) in 30 UHT flavored milk samples in Karaj, Alborz province, Iran. High-performance liquid chromatography (HPLC) was applied to analyze AFM1 in the samples. The results showed that aflatoxin M1 was detected in all the UHT flavored milk samples, the AFM1 concentration ranged from 0.015 to 0.14 µg/L. Also, 10 samples (33.3%) w...

Thirty five samples were collected from seven types of milk powder in Mosul city markets, and surveyed for the presence of aflatoxin M1 (AFM1), by using Enzyme Linked Immunosorbent Assay (ELISA) technique. Analytical results showed that 82.8% of the samples were contaminated with AFM1. The incidence of AFM1 in Al-mudhish, Angolac, Dielac, Lona, Nido, Melgro and Multi samples were 40, 50, 80, 10...

Aflatoxin M1 (AFM1) is a potent carcinogen, teratogen and mutagen found in the milk when lactating animals consume feed contaminated with aflatoxin B1 (AFB1). In the present study, the contamination of AFM1 was evaluated in the milk supply chain of the province of Sindh, Pakistan. For the broader profiling of targeted toxin, enzyme-linked immunosorbent assay (ELISA) was used for the determinati...

Aflatoxin M1 (AFM1) is found in milk and other excretion products after aflatoxin B1 intake. AFM1 is carcinogenic to humans, and known levels of dairy product contamination is important to understand the risks to which the population is exposed. The occurrence of AFM1 was evaluated in 42 milk samples commercialized in Londrina, Paraná State, Brazil and this rate of occurrence was used to estima...

An improved monoclonal antibody immunoaffinity chromatography/high-pressure liquid chromatography/ fluorescence detection method was developed to measure aflatoxin (AF) exposure by quantifying AFM1 in human and rat urine samples. Analysis of different amounts of various AF metabolites showed that the immunoaffinity resin was highly selective for aflatoxin B1 (AFB1), AFB2, and AFM1. Recovery of ...

OBJECTIVES Aflatoxins as a highly toxic group of mycotoxins are present in the environment and foodstuff. These have been reported to cause serious health problems in humans. Since aflatoxin M1 (AFM1) is excreted into breast milk, investigating the exposure of infants to AFM1 is of special concern. METHODS In the present study, breast milk samples were collected from 85 lactating mothers in I...

An experiment was carried out to study the excretion of aflatoxin M1(AFM1) in milk of three goats fed a single dose (0.8mg/head) of pure aflatoxin B1 (AFB1). The values of AFM1 concentration excreted in milk was highly variable among goats, even if the pattern of excretion over time was very similar among the three animals. AFM1 was first detected at the milking performed 1h after the AFB1 admi...