Acetyl-CoA carboxylase (ACC) catalyzes the first step of fatty acid biosynthesis, the synthesis of malonyl-CoA from acetyl-CoA using ATP and bicarbonate. In Escherichia coli and most other bacteria, ACC is composed of four subunits encoded by accA, accB, accC, and accD. Prior work from this laboratory showed that the in vivo expression of the accBC operon had a strikingly nonlinear response to ...

Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to any known protein. Expression of accB and accE in Escherichia coli and subsequen...

Transcription of the biotin (bio) biosynthetic operon of Escherichia coli is negatively regulated by the BirA protein, an atypical repressor protein in that it is also an enzyme. The BirA-catalyzed reaction involves the covalent attachment of biotin to AccB, a subunit of acetyl coenzyme (acetyl-CoA) carboxylase. The two functions of BirA allow regulation of the bio operon to respond to the intr...

We have cloned a DNA fragment from a genomic library of Myxococcus xanthus using an oligonucleotide probe representing conserved regions of biotin carboxylase subunits of acetyl coenzyme A (acetyl-CoA) carboxylases. The fragment contained two open reading frames (ORF1 and ORF2), designated the accB and accA genes, capable of encoding a 538-amino-acid protein of 58.1 kDa and a 573-amino-acid pro...

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. moluccana have various medical and commer...

A new severe plastic deformation (SPD) based technique entitled Accumulative channel-die compression bonding (ACCB) is proposed for the fabrication of high strength multi-layered Al/Cu composites for the first time. In order to primarily demonstrate the capabilities of ACCB in the fabrication of metal matrix composites (MMCs), AA 1050 and pure Cu strips were processed. The primary Al/Cu sandwic...

In this paper, the Accumulative Channel-die Compression Bounding (ACCB) method is investigated as a new method of severe plastic deformation to produce Nano-crystalline bulk metals on the basis of pressing in a channel mold. Aluminum as One of the most usable metal in industry is processed using this method. Analyzing the processed samples show that after four passes of ACCB method, the ultimat...

in this paper, the accumulative channel-die compression bounding (accb) method is investigated as a new method of severe plastic deformation to produce nano-crystalline bulk metals on the basis of pressing in a channel mold. aluminum as one of the most usable metal in industry is processed using this method. analyzing the processed samples show that after four passes of accb method, the ultimat...

The genes encoding two subunits of acetyl coenzyme A carboxylase, biotin carboxyl carrier protein, and biotin carboxylase have been cloned from Bacillus subtilis. DNA sequencing and RNA blot hybridization studies indicated that the B. subtilis accB homolog which encodes biotin carboxyl carrier protein, is part of an operon that includes accC, the gene encoding the biotin carboxylase subunit of ...

Polimer biodegradable dipandang sebagai solusi potensial bagi lingkungan yang dihasilkan oleh limbah plastik. PLA adalah salah satu biopolimer paling menjanjikan karena monomer diproduksi dari bahan baku terbarukan sehingga tidak beracun. Polikaprolakton (PCL) merupakan polyester ideal bersifat non toksid, biodagradable dan memiliki sifat termal baik dengan suhu degradasi sebesar ±4000C. Selain...