نتایج جستجو برای: AAPH

تعداد نتایج: 218  

2013
Rong-Rong He Yan Li Xiao-Di Li Ruo-Nan Yi Xiao-Yu Wang Bun Tsoi Kenneth Ka Ho Lee Keiichi Abe Xuesong Yang Hiroshi Kurihara

It is now well established that the developing embryo is very sensitive to oxidative stress, which is a contributing factor to pregnancy-related disorders. However, little is known about the effects of reactive oxygen species (ROS) on the embryonic cardiovascular system due to a lack of appropriate ROS control method in the placenta. In this study, a small molecule called 2,2-azobis(2-amidinopr...

2016
Maria D. Moreira Gomes Giovanna M. C. Carvalho Natalia V. Casquilho Andressa C. P. Araújo Samuel S. Valença Jose H. Leal-Cardoso Walter A. Zin

Recently, several studies have reported that respiratory disease may be associated with an increased production of free radicals. In this context, 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) is a free radical-generating compound widely used to mimic the oxidative stress state. We aimed to investigate whether AAPH can generate lung functional, inflammatory, histological and biochemical...

Gholamreza Karimi, Mohammad Hassanzadeh Soghra Mehri

       The oxidative hemolysis of rat erythrocytes induced by 2,2’-azobis–(2-amidinopropane) (AAPH) and its inhibition by rosemary essential oil was studied. Different concentrations (0.178, 0.357, 0.534 and 0.712 ml/ml) of the essential oil showed no significant hemolysis compared to phosphate buffer solution. AAPH (25 mM and 50 mM) induced hemolysis in a time-dependent manner. Diff e r e n t ...

Journal: :Bioscience, biotechnology, and biochemistry 2004
Teruyuki Usui Satomi Shizuuchi Hirohito Watanabe Fumitaka Hayase

Blue-M1 is a blue pigment formed from xylose and glycine in the Maillard reaction. Previous work revealed that Blue-M1 scavenged hydroxyl radicals, and prevented the autoxidation of linoleic acid in vitro. We investigated the protective effect of Blue-M1 for 2,2'-azobis(2-amidino-propane)dihydrochloride (AAPH)-induced toxicity in COS-1 cells. COS-1 cells were cultured in AAPH containing DMEM me...

Journal: :Journal of enzyme inhibition and medicinal chemistry 2009
Nikolai L Chepelev Joshua D Bennitz James S Wright Jeffrey C Smith William G Willmore

In mammals, aging is linked to a decline in the activity of citrate synthase (CS; E.C. 2.3.3.1), the first enzyme of the citric acid cycle. We used 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), a water-soluble generator of peroxyl and alkoxyl radicals, to investigate the susceptibility of CS to oxidative damage. Treatment of isolated mitochondria with AAPH for 8-24 h led to CS inactivat...

2010
Chiaki Kusumoto Tomoyo Kinugawa Hitoshi Morikawa Mari Teraoka Tadashi Nishida Yoshikazu Murawaki Kazuo Yamada Tatsuya Matsura

Reduced coenzyme Q(10) (CoQ(10)H(2)) is known as a potent antioxidant in biological systems. However, it is not yet known whether CoQ(9)H(2) could act as an antioxidant in human cells. The aim of this study is to assess whether exogenously added CoQ(9) can protect human liver cells against injuries induced by a water-soluble radical initiator, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH...

2013
M. Gokila Vani K. J. Senthil Kumar Jiunn-Wang Liao Shih-Chang Chien Jeng-Leun Mau Shen-Shih Chiang Chin-Chung Lin Yueh-Hsiung Kuo Sheng-Yang Wang

In this study, we investigated the cytoprotective effects of antcin C, a steroid-like compound isolated from Antrodia cinnamaomea against AAPH-induced oxidative stress and apoptosis in human hepatic HepG2 cells. Pretreatment with antcin C significantly protects hepatic cells from AAPH-induced cell death through the inhibition of ROS generation. Furthermore, AAPH-induced lipid peroxidation, ALT/...

2011
G Karimi M Aghasizadeh M Razavi E Taghiabadi

BACKGROUND AND THE PURPOSE OF THE STUDY It has been shown that Nigella sativa L. and Portulaca oleracea L. have many antioxidant components. In the present study, the cytoprotective effect of ethanolic and aqueous extracts of N.sativa and P.oleracea against hemolytic damages induced by free radical initiator, AAPH [2, 2' azobis (2- amidinopropane) hydrochloride] was evaluated. METHODS Hemolys...

Journal: :Journal of agricultural and food chemistry 2000
T Yokozawa E J Cho Y Hara K Kitani

This study investigated the antioxidative activity of green tea extract, and a green tea tannin mixture and its components, under conditions of radical generation using the hydrophilic azo compound, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) to generate peroxyl radicals at a constant and measurable rate in the cultured renal epithelial cell line, LLC-PK(1), which is susceptible to oxi...

Journal: :Folia morphologica 2009
J Antosiewicz J H Spodnik M Teranishi A Herman-Antosiewicz Ch Kurono T Soji M Woźniak A Borkowska T Wakabayashi

There is general agreement that oxidative stress may induce apoptotic and necrotic cell death. Recently it has been shown that NADH can be considered an important antioxidant as it reacts with peroxyl and alkoxyl radicals under in vitro conditions. Therefore, in the present study we hypothesized that an increase in intracellular NADH using specific substrates will protect RL-34 cells against cy...

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