myocardial ischemia (mi) resulting in infarction is an important cause of mortality and morbidity worldwide. acute ischaemia rapidly impairs myocardial contractile function. myocardial dysfunction persisting for several hours after transient non-lethal ischaemia, eventually resulting in full functional recovery is termed as myocardial stunning. hibernation is now thought to be the consequence o...

BACKGROUND Phosphatidylserine (PS) is a phospholipid normally located in the inner leaflet of the cell membrane. PS is translocated from the inner to the outer leaflet of the plasma membrane during the early stages of apoptosis and in necrosis. In cell and animal studies, reversible PS externalisation to the outer membrane leaflet has been observed in viable cells. Hence, PS markers have been p...

Non-invasive in vivo molecular imaging using radionuclides is based on the Radiotracer Principle of G. von Hevesy and the magic bullet concept by Ehrlich (1990). Both principles require administration of a radioactive tracer (a molecule labelled with a radioactive isotope which when injected into a living object can be traced by external radiation detection devices) and require that the quantit...

In recent years, several radiopharmaceuticals have been developed for imaging regional cerebral blood flow (rCBF) such as 75Se labelled PIPSE and MOSE, ²º¹TI labelled DDC and 99mTc labelled HMPAO. HMPAO with two asymmetric carbon atoms (At the 3 and 9 positions), produces d,1 and meso isomers, only one of which is retained in the brain. Rapid intracellular conversion of 99mTc-d,1-HMPAO to...

OBJECTIVE The purpose of this study was to develop an imaging technique to measure and monitor tumor cells undergoing programmed death caused by radiation and chemotherapy using 99mTc-EC-annexin V. Annexin V has been used to measure programmed cell death both in vitro and in vivo. Assessment of apoptosis would be useful to evaluate the efficacy and mechanisms of therapy and disease progression ...

in recent years, several radiopharmaceuticals have been developed for imaging regional cerebral blood flow (rcbf) such as 75se labelled pipse and mose, ²º¹ti labelled ddc and 99mtc labelled hmpao. hmpao with two asymmetric carbon atoms (at the 3 and 9 positions), produces d,1 and meso isomers, only one of which is retained in the brain. rapid intracellular conversion of 99mtc-d,1-hmpao to hydro...

A simple technique is described for producing high-quality functional images of regional ventilation during physiological tidal breathing of the inert gas 81mKr. These images are quickly obtained on a gamma-camera without the need of computerized systems for data acquisition and display and are directly comparable with those of perfusion obtained with 99mTc-labelled macroaggregates. The short t...

context inherited and sporadic medullary thyroid cancer (mtc) is an uncommon and medically challenging malignancy. even if the extent of initial surgery is deemed adequate, the recurrence rate remains high, up to 50% in most series. measurement of serum calcitonin is important in the follow-up of patients with mtc, and reliably reflects the existence of the disease. evidence acquisition there i...

UNLABELLED There is increasing evidence that cell death after myocardial ischemia and reperfusion may begin as apoptosis rather than necrosis. To determine the time course, location, and extent of this process, we studied groups of rats after a 20-min interval of coronary occlusion and reperfusion. METHODS After thoracotomy, the left coronary artery was occluded for 20 min. After release and ...

A convenient strategy to radiolabel a hydrazinonicotonic acid (HYNIC)-derived tetrazine with 99mTc was developed, and its utility for creating probes to image bone metabolism and bacterial infection using both active and pretargeting strategies was demonstrated. The 99mTc-labelled HYNIC-tetrazine was synthesized in 75% yield and exhibited high stability in vitro and in vivo. A trans-cyclooctene...