We have investigated the intracellular distribution and mobility of the glycolytic enzyme enolase, using functional fluorescent analogs labeled with the succinimidyl esters of carboxyfluorescein (F1-enolase) and carboxytetramethylrhodamine (Rh-enolase) In contrast to aldolase, neither native enolase nor labeled enolase gelled filamentous actin (F-actin), as measured by falling-ball viscometry, ...

Undiluted cytosolic extracts were prepared from fast glycolytic muscle tissue of white perch (Morone americanus). Diffusion coefficients (D) through the cytosol preparations were estimated in vitro for a series of selected low molecular weight compounds using an experimental diffusion chamber. Determinations were made at 5 degrees and 25 degrees C to assess thermal sensitivity of the process. N...

The lateral mobility of alkaline phosphatase (AP) in the plasma membrane of osteoblastic and nonosteoblastic cells was estimated by fluorescence redistribution after photobleaching in embryonic and in tumor cells, in cells that express AP naturally, and in cells transfected with an expression vector containing AP cDNA. The diffusion coefficient (D) and the mobile fraction, estimated from the pe...

ABSTRACT We apply the stochastic model of iron transport developed by Rebusco et al. (2005) to the Centaurus cluster. Using this model, we find that an effective diffusion coefficient D in the range 2× 1028− 4× 1028 cm2s−1 can approximately reproduce the observed abundance distribution. Reproducing the flat central profile and sharp drop around 30− 70kpc, however, requires a diffusion coefficie...

We have investigated the effects of glucocorticoid treatment on lipid lateral diffusion and cholesterol:phospholipid ratios in the plasma membranes of the lipopolysaccharide (LPS)-responsive murine B-cell leukemia (BCL1). Exposure to LPS for 24 h at concentrations of 50 micrograms/ml, 100 micrograms/ml, and 250 micrograms/ml caused a 42% reduction in the lateral diffusion of the lipid probe 3,3...

In this research, the diffusion coefficient of phosphate ions in agarose-citric acid diffusive gel has been studied. The layer was prepared by mixing 1.5% agarose solution with addition citric solutions at various concentration. casted between two glass plates which were separated using a spacer 0.1 cm thickness and clamped together three sides. Gel formed for 15 minutes an oven 40oC. then calc...

I have proposed a bottom-up technology utilising irradiation with active beams, such as electrons and ions, to achieve nanostructures size of 3–40 nm. This can be used nanotechnology that provides the desired structures, materials, phases at positions. Electron beam metastable θ-Al2O3, more than 1019 e/cm2s in transmission electron microscope (TEM), enables production oxide-free Al nanoparticle...

Modification of a glassy carbon (GC) electrode surface by adhered microparticles of zinc oxide (ZnO) using electrochemical performance of microparticles of ZnO/GC electrode shows excellent electrocatalytic activity towards the oxidation of ascorbic acid in 0.1 M KH2PO4 electrolyte solution by cyclic voltammetry (CV). This paper seeks to critically examine the modification of GC electrode with Z...

Scanning tunneling microscopy shows that large two-dimensional Ag clusters on Ag(100) can diffuse. The value of the diffusion coefficient at room temperature is of order 10-17 cm2s-1 and varies little, if at all, with cluster size in the range studied, 100 to 720 atoms per cluster. This weak variation rules out periphery diffusion as the main mechanism of cluster diffusion, suggesting instead t...

We have compared the lateral diffusion of intact transmembrane proteins, wild-type H2L d antigens, with that of mutants truncated in the cytoplasmic domain. Diffusion coefficients and mobile fractions were similar for all molecules examined, from wild-type L d antigens with 31 residues on the cytoplasmic side of the plasma membrane to mutants with only four residues in the cytoplasmic domain. T...