A series of new 2,4,6-trisubstituted-s-triazine was synthesized, assessed for antimicrobial activity, and characterized by FTIR, (1)HNMR, (13)CNMR, and elemental analysis. The tested compounds, 4d, 4g, 4h, 4k, and 4n, have shown considerable in vitro antibacterial efficacy with reference to the standard drug ciprofloxacin (MIC 3.125 μgmL(-1) against B. subtilis, E. coli, and K. pneumoniae). It ...

Pancreatic α-amylase inhibitors offer an effective strategy to lower the levels of post prandial hyperglycemia via control of starch breakdown. Eleven Ayurvedic Indian medicinal plants with known hypoglycemic properties were subjected to sequential solvent extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory potential on pancreatic α-amylase. Analysis...

Objective: Broussonetia papyrifera is widely used in Chinese traditional medicine. The current study was carried out to evaluate cytotoxic activity of methanolic extracts of leaf, bark and fruit on MCF-7, HeLa and HepG2 cell lines. Methods: The cytotoxic activity was determined as percentage of growth inhibition and cytotoxicity by Trypan blue dye exclusion assay and MTT assay respectively. Res...

A facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are...

A simple and sensitive method for the spectrophotometric determination of risperidone (RIS) is proposed. The method is based on the oxidation of the drug using chloramine-T (CAT) and the excess oxidant is determined by either xylene cyanol FF (XCFF) or malachite green (MG), the absorbances of which are measured at 612 or 619 nm for RIS-XCFF and RIS-MG respectively. Under the proposed optimum co...

A simple and rapid spectrophotometeric procedure has been established for quantization of Ni(II) at trace levels. The reagent isonicotinohydroxamic acid (INHA) reacts with nickel(II) at pH < 10 and form a 1:2 lemon green complex. The complex has a maximum absorption at 610 nm with the molar absorptivity of 1.44x10 L molcm. Beer’s law is obeyed over the range of 0.32-4.80 μgmL. The Sandell’s sen...

Citrullus colocynthis is an herbaceous perennial wild species of the Cucurbitaceae family, used in traditional medicine treatment diabetes mellitus. In present work, C. seed oils have been assessed for their fatty acids profile and concentration by Gas Chromatography–Mass Spectrometry (GC-MS), while oil protein extracts were investigated respect to amino acid compositions using Ultra High-Perfo...

New UPLC method was developed for determination of sitagliptin and metformin using Symmetry C18 column (100 mm × 2.1 mm, 2.2 μm) and isocratic elution (methanol 20%), pH (3.5) as a mobile phase. The ultraviolet detector was operated at 220 nm and the column temperature was 50°C. Linearity parameters were acceptable over the concentration ranges of 2-12 μgml(-1) and 5-35 μgml(-1) for sitagliptin...

A stability-indicating high-performance liquid chromatographic (HPLC) method was developed with short run time and validated for the assay of process related impurities of pantoprazole in bulk form. Resolution of drug, its potential impurities and degradation products were achieved on a Hypersil ODS column utilizing a gradient with 0.01 M phosphate buffer of pH 7 and acetonitrile as eluent, at ...

The phospholipids content of Cyperus esculentus tubers were identified by both HPTLC and/or HPLC techniques. The identified phospholipid classes were Phosphatidic acids, Phosphatidylinositol, Phosphatidylethanolamines, Phosphatidylcholine, L-α Phosphatidyl DL-glycerol, Cardiolipin, and L-α-Lysophosphatidylcholine. The hydrolysis of total phospholipids showed that four fatty acids were attached ...