We report the effect of Fab' (anti-60k) to a 60,000 mol wt gelatin binding domain of fibronectin (1981, J. Biol. Chem. 256:5583) on diploid fibroblast (IMR-90) extracellular fibronectin and collagen organization. Anti-60k Fab' did not inhibit IMR-90 attachment or proliferation in fibronectin-depleted medium. Fibroblasts cultured with preimmune Fab' deposited a dense extracellular network of fib...

The spliceosomal cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle, interacting with homologous sequences in the proteins U4/U6-60K and hPrp18 during pre-mRNA splicing. We determined the crystal structure of the complex comprising cyclophilin H and the cognate domain of U4/U6-60K. The 31 amino acid fragment of U4/U6-60K is bound to a region remote...

Cowpea mosaic virus (CPMV) replicates in close association with small membranous vesicles that are formed by rearrangements of intracellular membranes. To determine which of the viral proteins are responsible for the rearrangements of membranes and the attachment of the replication complex, we have expressed individual CPMV proteins encoded by RNA1 in cowpea protoplasts by transient expression ...

In cowpea protoplasts infected with cowpea mosaic virus, a bottom-component (B) RNA-encoded 60-kilodalton (60K) polypeptide is synthesized, which is membrane-bound and represents the direct precursor to the genome-bound protein VPg. The relationship between this VPg precursor and other B-RNA-encoded polypeptides was studied. Digestion of the B-RNA-encoded 170K and 84K polypeptides with Staphylo...

We are faced with an unprecedented production in scholarly publications worldwide. Stakeholders the digital libraries posit that document-based publishing paradigm has reached limits of adequacy. Instead, structured, machine-interpretable, fine-grained knowledge as Knowledge Graphs (KG) is strongly advocated. In this work, we develop and analyze a large-scale structured dataset STEM articles ac...

An x-ray detector for a solar axion search was developed. The detector is operated at 60K in a cryostat of a superconducting magnet. Special care was paid to microphonic noise immunity and mechanical structure against thermal contraction. The detector consists of an array of PIN photodiodes and tailor made preamplifiers. The size of each PIN photodiode is 11 × 11 × 0.5 mm and 16 pieces are used...

The synthesis and accumulation of Chlamydia trachomatis outer membrane proteins within infected HeLa 229 host cells were monitored by assessing the uptake of [35S]cysteine into chlamydial proteins during the 48-h growth cycle of a lymphogranuloma venereum strain, L2/434/Bu. Synthesis of the major outer membrane protein, a protein that accounts for about 60% of the outer membrane protein mass of...

We prove that two independent continuous-time simple random walks on the infinite open cluster of a Bernoulli bond percolation in the lattice Z meet each other infinitely many times. An application to the voter model is also discussed. 2000 MR subject classification: 60K