Acetylcholinesterase (AChE; EC 3.1.1.7) plays a crucial role in the rapid hydrolysis of the neurotransmitter acetylcholine, in the central and peripheral nervous system and might also participate in non-cholinergic mechanism related to neurodegenerative diseases. Alzheimer's disease (AD) is a neurodegenerative disorder characterized by a progressive deterioration of cognitive abilities, amyloid...

Clipping to sinus rhythm: cardioversion of atrial fibrillation by a thoracoscopic left atrial appendage occlusion Vedran Velagić*, Gian-Battista Chierchia, and Mark La Meir Centre for Cardiovascular Diseases, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, 101 Laarbeeklaan, 1090 Brussels, Belgium * Corresponding author. Tel: +385 91 7929284; fax: +322 477 6851, E-mail address: vvel...

Peroxisomes of higher eukaryotes, glycosomes of kinetoplastids, and glyoxysomes of plants are related microbody organelles that perform differing metabolic functions tailored to their cellular environments. The close evolutionary relationship of these organelles is most clearly evidenced by the conservation of proteins involved in matrix protein import and biogenesis. The glycosome can be viewe...

A simple and effective system has been developed from which a number of Plasmodium falciparum dihydrofolate reductase (pfDHFR) mutants conferring resistance to antifolates were randomly generated and characterized. The system exploited error-prone PCR to generate random mutations in the pfDHFR. Using the synthetic gene encoding for wild-type and quadruple mutant (N51I+C59R+S108N+I164L) pfDHFRs ...

To elucidate the process of transcription in the kinetoplastids and to aid in the purification of transcription factors, we have developed a transcriptionally-competent nuclear extract from Leishmania tarentolae for the study of the spliced leader (SL) RNA gene. The extract was competent to transcribe a tagged SL RNA gene. The in vitro SL RNA transcripts initiated accurately and their synthesis...

Laurent Pfister,1* Jeffrey J. McDonnell,2,3 Christophe Hissler1 and Lucien Hoffmann1 1 Centre de Recherche Public—Gabriel Lippmann, Department Environnement et Agro-biotechnologies, 41 rue du Brill, L-4422 Belvaux, Luxembourg 2 Department of Forest Engineering, Oregon State University, 015 Peavy Hall, Corvallis, OR 97331-5706, USA 3 School of Geosciences, University of Aberdeen, Aberdeen AB243U...

Center for Evolutionary Functional Genomics, The Biodesign Institute, Arizona State University, Tempe, AZ 85287-5301 School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501 Department of Electrical Engineering, Ira A. Fulton School of Engineering, Arizona State University, Tempe AZ 85287-5706 Department of Computer Science and Engineering, Ira A. Fulton School of Engineering, Ar...

*Toxicology and Molecular Biology Branch, ‡Pathology and Physiology Research Branch, and §Biostatistics Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, West Virginia 26505; †Ankara University School of Pharmacy, Department of Toxicology, 06100 Ankara, Turkey; and \BRT-Burleson Research Technologies, Inc., 5706 ...