conclusions results of the current study indicated that aconitase was not a suitable target to design new anti-fungal drugs that selectively block this enzyme. materials and methods candida krusei (atcc: 6258) aconitase gene was determined by 5’rapid amplification of cdna ends (race) method and degenerate polymerase chain reaction (pcr) and analyzed using bioinformatics softwares. objectives th...

Mutation in the dystrophin gene results Duchenne Muscular Dystrophy (DMD), an X-linked fatal neuromuscular disorder. Dystrophin deficiency can be compensated by upregulation of utrophin, an autosomal homologue of dystrophin. But the expression of utrophin in adults is restricted to myotendinous and neuromuscular junctions. Therefore utrophin upregulation throughout the muscle fiber can only be ...

phytophthora root and crown rot of soybean is known as a destructive disease of soy-bean both through out the world and in iran. physiologic races of phytophthora soja were determined in this research and also a fast, accurate and simple method for inoculation of soybean to test race specific resistance. during the years　2001-4, infected soybean plants at different growth stages were collected ...

With the advent of techniques for gene isolation based on polymerase chain reaction (PCR), there is an increasing need for methods to complete missing cDNA parts, especially in the more difficult 5′ side. While library construction and screening is always a possibility, the process is time-consuming and does not promise isolation of complete cDNAs. For this reason, several PCR-based methods hav...

abstract to study virulence and molecular polymorphism variation of puccinia striiformis westend. f.sp.tritici eriks. (yellow rust disease agent) in iran, 86 isolates were collected from all around the country. race determination was carried out according to johnson et al. (1972) method. in addition, isolates were analyzed by aflp method by four primer combinations . according to the results of...

Specific detection of mRNA cleavage by 5'RACE is the only method to confirm the knockdown of mRNA by RNA interference, but is rarely reported for in vivo studies. We have combined 5'-RNA-linker-mediated RACE (5'-RLM-RACE) with real-time PCR using a molecular beacon to develop a rapid and specific method termed MBRACE, which we have used to detect small-interfering RNA (siRNA)-induced cleavage o...