The zona pellucida (ZP) in a majority of species consists of three antigenic families with wide inter species cross reactivity. The glocoproteins of heat solubilized goat zona pellucida (gZP) were isolated into three families by high performance liquid chromatography on a gel ®ltration column. Their molecular identities were 180, 95 and 75 kDa for gZP1, gZP2 and gZP3 respectively. However under...

Previously, we produced a fertilization-blocking monoclonal antibody (MAb-5H4) to find a candidate peptide for a contraceptive vaccine. MAb-5H4 recognized a linear amino acid sequence of ZPA (No. 50-67) in pigs, humans and rabbits. In the present study, 18mer peptides corresponding to the sequence were conjugated with diphtheria toxoid as a carrier protein before immunization in rabbits. All th...

The bovine papillomavirus type 1 (BPV-1) E2 protein is the master regulator of papillomavirus replication and transcription. We have raised a panel of monoclonal antibodies (MAbs) against the BPV-1 E2 protein and used them to probe the structure and function of the protein. Five MAbs reacted with linear epitopes, and four MAbs recognized conformation-dependent epitopes which mapped within the C...

To process silage, rye is usually removed before the heading stage but the rye biomass increased up to 30% after the heading stage. However, after the heading stage, lignification rapidly accelerated and it resulted in a poor NDF digestibility problem. This has led to a demand for a strong fibrinolytic enzyme-producing probiotic for rye silage. The Gram-positive Leuconostoc holzapfelii 5H4 was ...

To study the neutralizing epitopes of influenza B virus Yamagata group strains, two monoclonal antibodies (mAbs) were used to select escape mutants of the virus. mAbs 5H4 and 3A12 were found to react with B/Yamagata group strains in haemagglutination inhibition and neutralization tests; no reactivity with B/Victoria group strains was observed. Most of the mutants reacted poorly to polyclonal fe...

Département de physiologie et biophysique, Institut de pharmacologie de Sherbrooke, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC, Canada, Hatos Center for Neuropharmacology, David Geffen School of Medicine, University of California in Los Angeles, Los Angeles, CA, USA Correspondences Louis Gendron, Department of Physiology and Biophysics, Faculty of ...

We report the first definitive Nusselt number scale of thermal boundary layers from curved surfaces characterized by proposed non-dimensional curvature parameter ξ = R0/(HRa−1/4), where R0 denotes radius a surface, H corresponding finite height, and Ra global Rayleigh virtual reference layer on vertical flat surface. The is given Nu ∼ ξ−1/5Ra1/4 in which Ra1/4 for surface case, revealing that c...

Mixed manganese sandw ich complexes containing a silyl-substituted cyclopentadienyl ring, e. g. (rj5 -C5 H4-R)Mn(7/6-C6H6) (3a c) and (775-C 5H4-R)M n(r/6-C6H5-Ph) (4a c); (R = SiM e3 (a), Si2M e_5 (b) and SiM e^'Bu (c)), were obtained in low yield via interm ediates {(r^-CsH*R)M nCl} and their reaction with phenyl G rignard reagents. Use o f the 4-trim ethylsilyl-phenyl m agnesium halide in th...