We made substantial advances in the implementation of a rapamycin-triggered heterodimerization strategy. Using molecular engineering of different targeting and enzymatic fusion constructs and a new rapamycin analog, Rho GTPases were directly activated or inactivated on a timescale of seconds, which was followed by pronounced cell morphological changes. As signaling processes often occur within ...

Sir, Since the initial reports of vancomycin-resistant enterococci (VRE) in the late 1980s, increasing numbers of such isolates have been identified in centres in both Europe and the U S A . , 2 In 1998, Fujita et al. were the first authors in Japan to describe the isolation of a vancomycin-resistant strain of Enterococcus faecium from a patient with a urinary tract infection. To date, 10 strai...

The concepts in this course will center on the physiology of membranes and of excitable cells. Please approach this field of study with the point of view that the cell membrane is a highly dynamic, structured, complex organelle, subject to the same physical laws as is any other structure. Cell membranes are probably responsible for more tasks than any other organelle. Such tasks include mainten...

The absorption, metabolism, and excretion of darapladib, a novel inhibitor of lipoprotein-associated phospholipase A2, was investigated in healthy male subjects using [C]-radiolabeled material in a bespoke study design. Disposition of darapladib was compared following single i.v. and both single and repeated oral administrations. The anticipated presence of low circulating concentrations of dru...

Endoplasmic reticulum (ER) stress induced by misfolded proteins has been implicated in Parkinson's disease (PD) pathogenesis. A malfunction of unfolded protein response (UPR) to ER stress can result in PD as well as other neurodegenerative diseases. Heat shock 70 kDa protein 5 (HSPA5) is one of the UPR chaperones reactive to ER stress to block the apoptotic process. HSPA5 promoter polymorphisms...

Address for Correspondence: Sanjiv J. Shah, MD, FAHA, FACC Rahul C. Deo, MD, PhD Division of Cardiology, Dept of Medicine Division of Cardiology, Dept of Medicine Feinberg Cardiovascular Research Institute Cardiovascular Research Institute Northwestern Univ Feinberg School of Medicine University of California, San Francisco 676 N. St. Clair St., Suite 600 555 Mission Bay Boulevard South Chicago...

UNLABELLED Human astrovirus (HAstV) is a leading cause of viral diarrhea in infants and young children worldwide. HAstV is a nonenveloped virus with a T=3 capsid and a positive-sense RNA genome. The capsid protein (CP) of HAstV is synthesized as a 90-kDa precursor (VP90) that can be divided into three linear domains: a conserved N-terminal domain, a hypervariable domain, and an acidic C-termina...

Background: Chlamydia trachomatis (CT) is a sexually transmitted pathogen that threatens reproductive health worldwide. This study aims to screen CT urogenital infection using cytology and molecular methods in women suffering infertility. Methods: In total, 415 infertility, attending Wad Madani Maternity Ho...

Using 68Ge : 14C dual-labeling, we investigated the absorption efficiency of diatom carbon for the cold water appendicularian Oikopleura vanhoeffeni. The absorption efficiency of bulk carbon (mean 5 67%) was not influenced by body size or ingestion rate. For the first time for a pelagic tunicate, food and feces were fractionated into their major biochemical constituents (i.e., low-molecular-wei...