lemon balm (melissa officinalis l.) is an herbaceous perennial plant of the lamiaceae family that has a wide edible and medicinal uses. in this research the potential of five different explants (nodal segments, shoot tip, cotyledon, hypocotyl and leaf segments) on ms mediums supplemented with different concentrations of 6-benzylaminopurine (bap) (2.2, 4.4 and 8.8 µm) alone or in combination wit...

Nodal explants of Phyla nodiflora (L.) Greene, were cultured on Murashige and Skoog (MS) medium supplemented with different combination and concentration of cytokinins and auxins for multiple shoots regeneration. The maximum numbers of shoots were found in MS medium supplemented with2.5 mgl-16-benzylaminopurine (BA) and 0.5 mgl-1 kinetin (KN). Elongated shoots were transferred to rooting ...

punicagranatum cv. malassaveh is one of the most desirable commercial cultivars in iran. in thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. for thispurpose, both liquid and agarmurashige and skoog (1962) (ms) medium supplemented with baat 2, 4.5, 9, 13 μm alone and in...

An efficient regeneration protocol was developed from shoot tip and nodal explants of Simarouba glauca DC, a promising biodiesel plant. Nodal explants appeared to have better regeneration capacity than shoot tip explants (40%) in the tested media. The highest regeneration frequency (90%) and shoot number (7.00 ± 1.00 shoots per explants) were obtained in nodal explants in Murashige and Skoog’s ...

Punicagranatum cv. Malassaveh is one of the most desirable commercial cultivars in Iran. In thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. For thispurpose, both liquid and agarMurashige and Skoog (1962) (MS) medium supplemented with BAat 2, 4.5, 9, 13 μM alone and in...

In vitro budinduction and shoot regeneration of Lantana camara L. was investigated using axillary nodal explants. Multiple shoot buds were induced on Murashige and Skoog (MS) media supplemented with 0, 2, 4 and 8 mg/l of 6-benzylaminopurine (BAP) or thidiazuron (TDZ) for proliferation and 0, 0.25 and 0.5 mg/l indole-3 butyric acid (IBA) for rooting. After 4 weeks, a maximum number of shoot buds...

the first successful and an efficient regeneration system on in vitro direct plant regeneration of agastache foeniculum was reported. the experiment were done in factorial design with three replications and explants were cultured in ms media fortified with different concentrations of bap and tdz (0, 2.2, 4.4, 8.8 μm) in combination with 3 levels of iaa (0, 1.1 and 2.2 μm). anova analysis showed...

A rapid and efficient in vitro propagation protocol of Acorus calamus L. an important endangered medicinal plant has been developed. Naturally grown rhizome segments were used as explants for initial culture. Surface sterilized rhizome segments were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations (0.5 mg l 3.0 mg l ) of cytokinins (BAP, Kn) and auxins (IAA, ...

In this study the effect of growth regulators in different concentrations were tested for shoot and root development micropropagation pomegranate with nodal explants. The explants collected morning, third node from tip. healthy plants which authenticated. For MS media was supplemented alpha-naphthalene acetic acid (NAA), indole-3-butyric-acid (IBA), 6-benzyl aminopurine (BAP) kinetin their comb...