The selective staining of nucleolar acidic proteins by silver dyes (AgNOR technique) is a promising tool to discriminate malignant cells from benign cells in tumour pathology. Until now, the existing methods to measure the distribution of the AgNOR proteins were performed in two-dimensional space. Confocal scanning laser microscopy (CSLM) and progress in computer sciences make it possible to im...

After the pioneering work by Ploton et al. [1] silver staining of nucleolar organizer regions (AgNOR) has been introduced into tumour pathology as a proliferation marker which is closely related to the rapidity of cell cycle [2]. Owing to wet autoclave pretreatment introduced by Öfner et al. [3] AgNOR analysis has now widely been standardized in tissue sections [4]. In cytological specimens, ho...

Study of AgNOR has been identified as a reliable indicator of cell proliferation and in turn, allows a clear distinction between benign, premalignant, and malignant epithelial changes. The aim of this study was to analyze and evaluate the application of AgNOR method as diagnostic tool in non-neoplastic and neoplastic lesions of endometrium. The study included evaluation of AgNOR staining on a t...

The quantitative distribution of AgNOR proteins represents a reliable index for predicting the clinical outcome of neoplastic patients. The rationale for the utilization of the AgNOR parameter in tumor pathology is based on the following observations: (1) the total quantity of AgNOR proteins evaluated in situ in human cancer cell lines by morphometric analysis is related to the rapidity of cell...

AIM To investigate the prognostic significance of DNA ploidy and silver stained nucleolar organizer regions (AgNOR), as well as their relation to the histological grade and Dukes' stages of colorectal carcinomas, and the relation of tumor cells proportion in the S-phase and Dukes' stage, histologic grade, DNA ploidy, or AgNOR count. METHODS DNA flow cytometric analysis and AgNOR were performe...

Nucleolar organizer regions identified by means of an argyrophilic technique (AgNOR) were quantified by digital image analysis in 81 patients with squamous cell carcinoma of the lung. Survival rate was modeled as function of AgNOR expression by Cox regression models controlled for staging, histologic grade, age, and sex. Our results indicate that AgNOR expression has a significant prognostic ro...

OBJECTIVE To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC). METHODS A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohis...

CONTEXT Only a few studies are available in the literature regarding the AgNOR (argyrophilic nucleolar organizer region) count in pancreatic adenocarcinoma but studies on the SAPA (subjective AgNOR pattern assessment) score are completely lacking. OBJECTIVE We attempted to estimate the AgNOR count and the SAPA score in carcinoma of the pancreatic head including periampullary tumors and to cor...

Discrimination of regenerative cirrhotic nodules of the liver (CN) from hepatocellular carcinoma (HCC) is sometimes difficult. We examined the utility of AgNOR staining in this context. Fifteen cases of HCC and 25 cases of CN were stained by AgNOR method and the mean AgNOR number for one hundred nuclei in each case was determined. There was a significant difference between the mean AgNOR c...