the aim of this study was to evaluate the effect of bovine serum albomin (bsa) in stallion semen extender in cool condition. after collection, semen samples were pooled and divided into four equal parts. each part was diluted with one of the following extenders: 1) extender without bsa (bsa-0), 2) extender containing 5 mg/ml bsa (bsa-5), 3) extender containing 10 mg/ml bsa (bsa-10), and 4) exte...

The purpose of this study was to investigate the effects of semen collection into tubes containing extender supplemented with BSA on the cryosurvival of goat spermatozoa. Semen was collected from two goats into empty tubes or tubes containing 10 ml extender supplemented with 0, 0.1, 1, or 5% BSA, and the washed spermatozoa were frozen as pellets in egg yolk-trehalose extender with the addition ...

The present work was designed to study the effect of different concentrations of bovine serum albumin (BSA) in extender on post-thaw quality of buffalo spermatozoa. Semen was collected from three buffalo bulls with artificial vagina (42°C) for three weeks (replicates). Qualifying semen ejaculate from each bull was split into five aliquots and diluted in tris-citric egg yolk extender containing ...

The present study aimed to compare the fertility of ewes intrauterinally inseminated with frozen-thawed semen using a soybean-based semen extender (AndroMed) with those of ewes intrauterinally inseminated with frozen-thawed semen using a Tris-based extender containing either egg yolk or BSA. Suffolk ewes (n=104) were treated with an intravaginal sponge containing 40 mg fluoroprogesterone acetat...

This experiment wascarried out to explore the effect of use of combinations of permeating cryoprotectant and non permeating cryoprotectant on thequality of frozen-thawed Beluga (Huso huso) spermas compared to the use of permeating cryoprotectant as a control. For this work methanol (MeOH) and dimethyl sulfoxide (DMSO)are used aspermeatingcryoprotectantsand bovine serum albuminas a non permeatin...

The aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºC for 0, 12, 24, 48 and 72 hrs after collection anddilution. A total of 57 ejaculates from 12 Bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following an...

the aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºc for 0, 12, 24, 48 and 72 hrs after collection anddilution. a total of 57 ejaculates from 12 bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following an...

Three (AB-3, AB-4 and AB-6) monoclonal antibodies (mAb) to bovine serum albumin (BSA) were derived and characterized for their physicochemical and immunological properties. AB-3 recognized an epitope distinct from epitopes recognized by AB-4 and AB-6 as determined by binding inhibition assay. AB-4 and AB-6 mAbs recognized similar but not identical epitopes on BSA. Based on the antigenic specifi...