it is important to identify the genetic diversity and phylogenic relationships for achieving desirable citrus cultivars. in present investigation rapd markers were used to determine genetic diversity among 29 citrus genotypes. from 19 used primers, 248 polymorphic bands were amplified. primers opa-07 and opa-05 (with 19 and 8 amplified bands), produced maximum and minimum polymorphic bands, res...

The current study was conducted at University of Kufa, Faculty Science, Biology Department for molecular identification four Eruca sativa L. cultivars (1-Turish 2-Syrian 3-Turkish 4-Eygyptian) using eleven Random Amplified Polymorphic DNA. Results indicate primers OPA-04, OPA-10, OPA-02, OPW-04, OPC-09,v and OPD-13 success in giving all a unique fingerprint .Highest number main bands , polymorp...

CURRENT SCIENCE, VOL. 81, NO. 3, 10 AUGUST 2001 248 by repeating the experiment at least once. A total of 34 decamer primers (series OPA, OPB etc.) from Operon Tech, CA, USA were screened using three representative genomic DNA samples of black pepper. Of these, 24 primers that yielded consistent and clear band patterns were selected for the final analysis of the 22 accessions used in the presen...

The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides ...

Escherichia coli O157:H7, an emerging cause of food-borne disease, has now been reported from several countries worlwide. Cattle and sheep are known as a principle reservoir of this agent. It is also prevalent in gastrointestinal tract of other animals like pigs, dogs, cats and birds. This agent can be transmitted to humans by food and water and even person to person contact. The random amplifi...

Random amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of primer, magnesium chloride, template DNA and Taq DNA polymerase with the objective of determining t...

Random amplified polymorphic DNA markers were used to evaluate the genetic diversity in a representative population of Terminalia pallida from six different geographical regions of chittoor district. 20 OPA primers were used for the RAPD analysis. A total of 33 bands yielded from 7 reproducible primers, with a mean of 4.28 amplified bands per primer. The plant species showed 69.5% polymorphism....

Genetic diversity of 12 lemon balm ecotypes was evaluated using agronomic traits as well as ISSR and RAPD markers. Results of analysis of variance showed high diversity among the studied ecotypes. Cluster analysis using UPGMA method grouped the ecotypes into three different groups based on the agronomic traits. Twelve ISSR primers created 106 polymorphic bands among the studied ecotypes. An ISS...

genetic diversity relationships of 50 isolates of cytospora schulzeri on apple from different parts of the semirom region were analyzed using 15 polymerase chain reaction (pcr) based markers, 7 random amplified polymorphic dnas (rapds) and 8 microsatellite primed polymerase chain reaction (mp-pcr). using 7 selected rapd primers 113 bands were generated, of which 81 bands were polymorphic (71.7%...

ziziphora tenuior, named kakooti in farsi, is one of the aromatic and medicinal plants of a wide distribution in iran. in this study, rapd molecular markers, (by utilization of 16 primers) was applied to determine the genetic diversity of 39 samples of ziziphora tenuior from 21 geographical regions in iran. a number of 100 polymorphic bands out of 110 amplified ones was produced and analyzed th...