Quantitative polymerase chain reactions (qPCR) based on real-time PCR constitute a powerful and sensitive method for the analysis of nucleic acids. However, in qPCR, the ability to multiplex targets using differently colored fluorescent probes is typically limited to 4-fold by the spectral overlap of the fluorophores. Furthermore, multiplexing qPCR assays requires expensive instrumentation and ...

Percutaneous Coronary Intervention Ventricular Septal Mass Formation After Retrograde Chronic Total Occlusion Print ISSN: 0009-7322. Online ISSN: 1524-4539 Copyright © 2013 American Heart Association, Inc. All rights reserved. is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231 Circulation doi: 10.1161/CIRCULATIONAHA.113.001476 2013;128:2167-2168 Circulation...

ISSN 2167-8421 print/ISSN 2167-9223 online © 2013 Informa Healthcare DOI: 10.3109/21678421.2013.826469 neuroinfl ammation (18,19) occur in ALS, although attempts to manipulate these events have not yet shown clear benefi ts in human ALS trials. Propofol has a short half-life; once infusion is stopped, its clinical effects last only minutes (3). For this reason, it is diffi cult to understand ho...

confluent monolayers of well-differentiated enterocyte-like cells with functional properties of transporting epithelia, and retains various influx and efflux transporters expressed in the intestine, such as H -coupled oligopeptide transporter 1 (PEPT1), P-glycoprotein (P-gp), multi-drug resistance proteins (MRPs), and breast cancer resistance protein (BCRP). Caco-2 cells have been widely used a...

The electronic structure and interband transitions of a-Al,O, have been studied using temperature-dependent vacuum ultraviolet spectroscopy from 5 to 43 eV, at temperatures ranging from 293 up to 2167 K, which is approaching the melting point of 2327 K. The energy range of the spectra spans the full range of interband electronic transitions. Kramers-Kronig analysis has been employed to recover ...

antibiotics with a complex composition and structure and is produced by certain strains of Bacillus licheniformis and Bacillus subtilis. It is mainly active against Gram-positive bacteria. Bc consists of a mixture of structurally similar polypeptides from 12 amino acids (AA). The most important and known component is Bc A (Fig. 1). Other important components are B1, B2 and B3 which together wit...