A novel amino acid derivative 3-(4-(1, 2, 4, 5-tetrazine-3-yl) phenyl)-2-aminopropanoic acid was synthesized in this study. The compound possessed better water-solubility and was synthesized more easily compared with the well-known and commercially available 3-(p-benzylamino)-1, 2, 4, 5-tetrazine. Tetrazine-containing amino acid showed excellent stability in biological media and might be used f...

The authors report the synthesis of some bioactive organic compounds which could be pharmacological interest using Dioscorea praehensilis tyrosinase immobilized on calcium-alginate beads. enzymatic these involved pyruvic acid as a source carbonyl groups, ammonia and catechol. Catechol was used substrate parent compound that supplied aromatic ring carbons for reactions. structures novel synthesi...

A hybrid Fv fragment of the dinitrophenyl-binding immunoglobulin A (IgA), MPOC315, has been generated by reconstituting a recombinant variable light chain (VL) produced in Escherichia coli with a variable heavy chain (VH) derived from the antibody. The Tyr34 residue of VL was substituted by His in order to introduce a catalytic imidazole into the combining site for the ester hydrolysis. The His...

Incorporating fluorescent amino acids by suppression of the TAG amber codon is a useful tool for site-specific labeling of proteins and visualizing their localization in living cells. Here we use a plasmid encoded orthogonal tRNA/aminoacyl-tRNA synthetase pair to site-specifically label firefly luciferase with the environmentally sensitive fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino...

A new Schiff base ligand (L) obtained by the condensation reaction of N-acetylaceto-otoluidineand 2-aminopropanoic acid (an amino acid), is used to synthesize four mononuclearcomplexes of [MLCl] and [ML2] types (where M = Cu(II) and Zn(II); L = Schiff base) bykeeping the metal and ligand ratio as 1:1 and 1:2 respectively. This ligand and its complexeshave been characterized on the basis of diff...