to assess the genetic relationships between 72 cultivated and 65 wild grapevines in west azerbaijan and in kurdistan, 19 ssr markers were employed. microsatellitic primer pairs amplified, a total of 163 alleles with an average of 8.6 alleles/locus (for cultivars) and 153 alleles with an average of 8.05 alleles/locus (for wild samples). the expected heterozigosity varied from 0.7 in cultivars to...

UNLABELLED PREMISE OF THE STUDY We developed nuclear simple sequence repeat (SSR) markers for the characterization of the biomass crop Miscanthus, especially M. sacchariflorus, M. sinensis, and M. ×giganteus, and tested for cross-species amplification. • METHODS AND RESULTS Twenty-nine SSR markers (di- and tetranucleotide repeats) were developed from DNA sequences obtained from 192 clones ...

Expressed sequence tags (ESTs) are a valuable source of molecular markers. To enhance the resolution of an existing linkage map and to identify putative functional polymorphic gene loci in hexaploid wheat (Triticum aestivum L.), over 260,000 ESTs from 5 different grass species were analyzed and 5418 SSR-containing sequences were identified. Using sequence similarity analysis, 156 cross-species ...

Among different classes of molecular markers, expressed sequence tags (ESTs) are a new resource for developing simple sequence repeat (SSR) functional markers for genotyping and genetic mapping in F1 hybrid populations of Vitis vinifera L. Recently, because of the availability of an enormous amount of data for ESTs in the public domain, the emphasis has shifted from genomic SSRs to EST-SSRs, wh...

Cultivated groundnut (Arachis hypogaea L.), is an important oil seed crop grown mostly in tropical and subtropical regions of the world. However, it suffers with narrow genetic diversity due to genetic bottlenecks such as the recent polyploidization and self pollinating nature of the crop. In such crop species, simple sequence repeats (SSRs), as compared to other marker systems such as restrict...

UNLABELLED PREMISE OF THE STUDY We developed microsatellite markers in the legume Lens culinaris from publicly available databases to enrich the limited marker resource available for the crop. • METHODS AND RESULTS Eighty-two primer sets were identified using expressed sequence sets of L. culinaris available in the National Center for Biotechnology Information (NCBI) database and were char...