Phylogenetic analysis based on A) the 16S ribosomal RNA gene (16SrRNA) and B) flagellin gene of Borrelia spp. Representative Borrelia spp. were used. GenBank accession numbers are in parentheses. For the amplification and direct sequencing of 16SrRNA, the 4 PCR primers rrs-F1 (5′-ATAACGAAGAGTTTGATCCTGGCT-3′), rrs-F2 (5′-GGTGTAAGGGTGGAATCTGTTG-3′), rrs-R3 (5′-TTTCGTGACTCAGCGTCAGT-3′), and rrs-R4...

Microbiological control is of crucial importance in the pharmaceutical industry regarding the possible bacterial contamination of the environment, water, raw materials and finished products. Molecular identification of bacterial contaminants based on DNA sequencing of the hypervariable 16SrRNA gene has been introduced recently. The aim of this study is to investigate the suitability of gene seq...

molecular isolation of biodegradable mycobacteria from water supplies of iranian hospitals background and objectives: some microorganisms, mainly members of two genera including pseudomonas and mycobacterium , were found to be capable of transforming and degrading of polluting agents. we herein report the isolation of a few mycobacteria with the ability to biodegrade organic and inorganic compo...

OBJECTIVE This study aims to use high throughput 16SrRNA gene sequencing to examine the bacterial profile of lymph node biopsy samples of patients with sarcoidosis and to further verify the association between Propionibacterium acnes (P. acnes) and sarcoidosis. METHODS A total of 36 mediastinal lymph node biopsy specimens were collected from 17 cases of sarcoidosis, 8 tuberculosis (TB group),...

rapid and accurate identification of microorganisms have a significant impact on strategies and fish health management programs. hence, in this study a duplex pcr assay based on the 16s rrna gene for simultaneous detection of aeromonas hydrophila rticc 1032 and escherichia coli rticc 2325 from pure cultures, and challenged fish tissues was performed and their results were compared with the resu...

Bacteriophage T7's gene 0.3, coding for an antirestriction protein, possesses one of the strongest translation initiation regions (TIR) in E. coli. It was isolated on DNA fragments of differing length and cloned upstream of the mouse dihydrofolate reductase gene in an expression vector to control the translation of this gene's sequence. The TIR's efficiency was highly dependent on nucleotides +...

In this study, Staphylococcus aureus was found to be the causative agent of furunculosis in 64 (27.5%) out 233 Iraqi patients presented with furunculosis. 16SrRNA gene located all isolates. Nevertheless, mecA and lukS-lukF genes were 60% 4% S. isolates, respectively. Interestingly, carrying isolates positive as well.

For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR wi...

We report a case of cavitary pneumonia caused by N. otitidiscaviarum in a man with diabetes mellitus and thrombocytopenia treated with systemic corticosteroid. Taxonomic identification involved phenotypic testing and molecular identification that was carried out by DNA sequencing of the 16SrRNA gene.