A molecular tool, focused on the mitochondrial Control Region (CR), was developed to discriminate the two hawthorn-feeding psyllid species, Cacopsylla melanoneura (Förster) and C. affinis (Löw), and to estimate their frequencies in mixed populations. The test was carried out in paired and single-tube amplifications and validated analysing 52 male specimens previously determined by morphological...

Polymerase chain reaction (PCR)-based detection strategies were adopted to examine the etiology and vectorship of pear decline in Taiwan (PDTW). 16S rDNA sequences were amplified from total DNAs prepared from PDTW-affected pear trees Pyrus serotina Rehd. cv. Hengshan and pear psyllid Cacopsylla chinensis using PCR. According to the sequence analyses, C. chinensis carried phytoplasmas of two 16S...

There have been efforts to overcome the problem in treatment of cancer using medicinal plants. It has been shown that Citrus essential oil of contains different terpens with antitumor activities. In this study we sought to determine the cytotoxicity of essential oils of Iranian Citrus limon (L.), C. medica (L.), C. sinsensis (L.) peels on cancer cell lines. Essential oils were prepared by hydr...